| Full text | |
| Author(s): |
Feitoza, Carla Q.
[1]
;
Semedo, Patricia
[1]
;
Goncalves, Giselle M.
[1]
;
Cenedeze, Marcos A.
[1]
;
Pinheiro, Helady S.
[2]
;
Pavao dos Santos, Oscar Fernando
[1]
;
Landgraf, Richardt Gama
[3]
;
Pacheco-Silva, Alvaro
[4, 1]
;
Saraiva Camara, Niels Olsen
[1, 5]
Total Authors: 9
|
| Affiliation: | [1] Univ Fed Sao Paulo, Div Nephrol, Expt & Clin Immunol Lab, Sao Paulo - Brazil
[2] Univ Fed Juiz de Fora, Div Nephrol, Juiz De Fora, MG - Brazil
[3] Univ Fed Sao Paulo, Dept Biol Sci, Sao Paulo - Brazil
[4] Hosp Israelita Albert Einstein, Inst Ensino & Pesquisa, Sao Paulo - Brazil
[5] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Transplantat Immunobiol Lab, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Inflammation Research; v. 59, n. 3, p. 167-175, MAR 2010. |
| Web of Science Citations: | 12 |
| Abstract | |
This work explored the role of inhibition of cyclooxygenases (COXs) in modulating the inflammatory response triggered by acute kidney injury. C57Bl/6 mice were used. Animals were treated or not with indomethacin (IMT) prior to injury (days -1 and 0). Animals were subjected to 45 min of renal pedicle occlusion and sacrificed at 24 h after reperfusion. Serum creatinine and blood urea nitrogen, reactive oxygen species (ROS), kidney myeloperoxidase (MPO) activity, and prostaglandin E2 (PGE(2)) levels were analyzed. Tumor necrosis factor (TNF)-alpha, t-bet, interleukin (IL)-10, IL-1 beta, heme oxygenase (HO)-1, and prostaglandin E synthase (PGES) messenger RNA (mRNA) were studied. Cytokines were quantified in serum. IMT-treated animals presented better renal function with less acute tubular necrosis and reduced ROS and MPO production. Moreover, the treatment was associated with lower expression of TNF-alpha, PGE(2), PGES, and t-bet and upregulation of HO-1 and IL-10. This profile was mirrored in serum, where inhibition of COXs significantly decreased interferon (IFN)-gamma, TNF-alpha, and IL-12 p70 and upregulated IL-10. COXs seem to play an important role in renal ischemia and reperfusion injury, involving the secretion of pro-inflammatory cytokines, activation of neutrophils, and ROS production. Inhibition of COX pathway is intrinsically involved with cytoprotection. (AU) | |
| FAPESP's process: | 06/03982-5 - Molecular aspects involved in leukocytes microbicidal and inflammatory activities in the lung |
| Grantee: | Sônia Jancar |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 07/07139-3 - The role of heme oxygenase 1 in different renal inflammatory process in experimental animal models |
| Grantee: | Niels Olsen Saraiva Câmara |
| Support Opportunities: | Research Projects - Thematic Grants |