| Full text | |
| Author(s): |
Botta, Sergio B.
[1]
;
Ana, Patricia A.
[2]
;
Santos, Moises O.
[3]
;
Zezell, Denise M.
[3]
;
Matos, Adriana B.
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Sao Paulo, Sch Dent, Dept Operat Dent, Sao Paulo - Brazil
[2] Fed Univ ABC, Biomed Engn Ctr Engn Modeling & Appl Social Sci, Santo Andre, SP - Brazil
[3] Ctr Lasers & Applicat, Energet & Nucl Res Inst, Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS; v. 100B, n. 4, p. 1009-1016, MAY 2012. |
| Web of Science Citations: | 20 |
| Abstract | |
This study aimed to evaluate the chemical interaction of collagen with some substances usually applied in dental treatments to increase the durability of adhesive restorations to dentin. Initially, the similarity between human dentin collagen and type I collagen obtained from commercial bovine membranes of Achilles deep tendon was compared by the Attenuated Total Reflectance technique of Fourier Transform Infrared (ATR-FTIR) spectroscopy. Finally, the effects of application of 35% phosphoric acid, 0.1M ethylenediaminetetraacetic acid (EDTA), 2% chlorhexidine, and 6.5% proanthocyanidin solution on microstructure of collagen and in the integrity of its triple helix were also evaluated by ATR-FTIR. It was observed that the commercial type I collagen can be used as an efficient substitute for demineralized human dentin in studies that use spectroscopy analysis. The 35% phosphoric acid significantly altered the organic content of amides, proline and hydroxyproline of type I collagen. The surface treatment with 0.1M EDTA, 2% chlorhexidine, or 6.5% proanthocyanidin did not promote deleterious structural changes to the collagen triple helix. The application of 6.5% proanthocyanidin on collagen promoted hydrogen bond formation. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012. (AU) | |
| FAPESP's process: | 05/51689-2 - Optics and Photonics Research Center at UNICAMP |
| Grantee: | Hugo Luis Fragnito |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 06/06746-0 - Methodology to evaluate the effects of laser irradiation in root caries prevention. |
| Grantee: | Patricia Aparecida da Ana |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 06/05684-1 - A contaminação com saliva influencia na adesão aos tecidos dentais? |
| Grantee: | Adriana Bona Matos |
| Support Opportunities: | Regular Research Grants |