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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of periodontal therapy on GCF cytokines in generalized aggressive periodontitis subjects

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de Lima Oliveira, Ana Paula [1] ; de Faveri, Marcelo [2] ; Gursky, Lauren Christine [3] ; Mestnik, Maria Josefa [2] ; Feres, Magda [2] ; Haffajee, Anne D. ; Socransky, Sigmund S. ; Teles, Ricardo Palmier [4, 5]
Total Authors: 8
[1] Univ Fed Minas Gerais, Sch Dent, Dept Periodontol, Belo Horizonte, MG - Brazil
[2] Univ Guarulhos, Dept Periodontol, Dent Res Div, Guarulhos, SP - Brazil
[3] Pontificia Univ Catolica Parana, Sch Dent, Curitiba, PR - Brazil
[4] Forsyth Inst, Dept Periodontol, Cambridge, MA 02142 - USA
[5] Harvard Univ, Sch Dent Med, Dept Oral Med Infect & Immun, Boston, MA 02115 - USA
Total Affiliations: 5
Document type: Journal article
Source: JOURNAL OF CLINICAL PERIODONTOLOGY; v. 39, n. 3, p. 295-302, MAR 2012.
Web of Science Citations: 50

Aim: To examine changes in levels of gingival crevicular fluid (GCF) cytokines, after periodontal therapy of generalized aggressive periodontitis (GAgP). Materials and Methods: Twenty-five periodontally healthy and 24 GAgP subjects had periodontal clinical parameters measured and gingival crevicular fluid (GCF) samples collected from up to 14 sites/subject. GCF samples were analysed using multiplex bead immunoassay for: GM-CSF, IFN-gamma, IL-10, IL-1 beta, IL-2, IL-6 and TNF-alpha. Aggressive periodontitis subjects were randomly assigned to either scaling and root planing (SRP) alone or SRP plus systemic amoxicillin (500 mg) and metronidazole (400 mg) 3 times a day for 14 days. Clinical parameters and GCF cytokines were re-measured 6 months after treatment. Differences over time were analysed using the Wilcoxon test and between groups using the Mann-Whitney test. Results: Significant reductions in GCF GM-CSF, IL-1 beta and the ratio IL-1 beta/IL-10 and increases in GCF IL-6 were detected after therapy. The mean change in GCF cytokines did not differ significantly between groups. Conclusions: Periodontal therapy improved GCF cytokine profiles by lowering IL-1 beta and increasing IL-10 levels. The reduction in GCF GM-CSF after therapy implicates this cytokine in the pathogenesis of GAgP. There was no difference between therapies in changes of GCF cytokines. (AU)

FAPESP's process: 07/56413-0 - Clinical and microbiological effects of amoxicillin and metronidazole systemic association to scaling and root planing in subjects with generalized aggressive periodontitis
Grantee:Marcelo de Faveri
Support type: Regular Research Grants