| Full text | |
| Author(s): |
Aissa, Alexandre Ferro
[1]
;
Pires Bianchi, Maria Lourdes
[1]
;
Ribeiro, Juliana Carvalho
[1]
;
Hernandes, Livia Cristina
[1]
;
de Faria, Adelia Ferreira
[2]
;
Mercadante, Adriana Zerlotti
[2]
;
Greggi Antunes, Lusania Maria
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Clin Toxicol & Bromatol Anal, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Campinas UNICAMP, Fac Food Engn, Dept Food Sci, Campinas, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Food and Chemical Toxicology; v. 50, n. 5, p. 1418-1424, MAY 2012. |
| Web of Science Citations: | 17 |
| Abstract | |
beta-Carotene (BC) is one of the natural pigments that is most commonly added to food; however, the utilization of BC is limited due to its instability. Microencapsulation techniques are commonly used because they can protect the microencapsulated material from oxidization. Nevertheless, the properties of the encapsulated compounds must be studied. We compared the antigenotoxic potential of pure and microencapsulated beta-carotene (mBC) in Wistar rats. Two doses of BC or mBC (2.5 or 5.0 mg/kg) were administered by gavage over a period of 14 days. The final gavage was followed by an injection of doxorubicin (DXR). After 24 h the animals were euthanized. The micronucleus test results showed that when both mBC and DXR were given, only the higher dose was antigenotoxic. The results of the comet assay show that when given in association with DXR, mBC had protective effects in the liver. The differences between the results obtained with BC and mBC suggest that possibly the carotenoid biodisponibility was modified by the process of microencapsulation. In conclusion, mBC does not lose its protective properties, but higher doses must be used to observe antigenotoxic effects. This is the first time that the genotoxicity and antigenotoxicity of a microencapsulated compound was evaluated in vivo. (C) 2012 Elsevier Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 05/59552-6 - Integrated evaluation of the stability and functional properties of natural pigments of foods |
| Grantee: | Adriana Zerlotti Mercadante |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 07/57702-6 - valuation of beta-carotene microencapsulated antimutagenic activity in cells of rats treated with antitumor doxorubicin using micronucleus test and comet assay |
| Grantee: | Alexandre Ferro Aissa |
| Support Opportunities: | Scholarships in Brazil - Master |