Association between diet and polymorphisms in individuals with statin-controlled dyslipidaemia grouped according to oxidative stress biomarkers

Author(s)

Botelho, Patricia Borges ^[1] ; Fioratti, Cyntia Okoshi ^[1] ; Rogero, Marcelo Macedo ^[2] ; Barroso, Lucia Pereira ^[3] ; Bertolami, Marcelo Chiara ^[4] ; Castro, Inar Alves ^[5]

Affiliation

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food & Expt Nutr, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Publ Hlth, Dept Nutr, BR-05508900 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Math & Stat, Dept Stat, BR-05508900 Sao Paulo - Brazil

^[4] Dante Pazzanese Inst Cardiol, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Alimentos & Nutr Expt, BR-05508900 Sao Paulo - Brazil

• Document type: Journal article
• Source: Brazilian Journal of Pharmaceutical Sciences; v. 48, n. 1, p. 39-49, JAN-MAR 2012.
• Web of Science Citations: 1

Abstract

The objective of this study was to investigate whether differences in diet and in single-nucleotide polymorphisms (SNPs) found in paraoxonase-1 (PON-1), 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR), cholesterol ester transfer protein (CETP) and apolipoprotein E (APOE) genes, are associated with oxidative stress biomarkers and consequently with susceptibility of low-density cholesterol (LDL) to oxidation. A multivariate approach was applied to a group of 55 patients according to three biomarkers: plasma antioxidant activity, malondialdehyde and oxidized LDL (oxLDL) concentrations. Individuals classified in Cluster III showed the worst prognoses in terms of antioxidant activity and oxidative status. Individuals classified in Cluster I presented the lowest oxidative status, while individuals grouped in Cluster II presented the highest levels of antioxidant activity. No difference in nutrient intake was observed among the clusters. Significantly higher γ- and δ-tocopherol concentrations were observed in those individuals with the highest levels of antioxidant activity. No single linear regression was statistically significant, suggesting that mutant alleles of the SNPs selected did not contribute to the differences observed in oxidative stress response. Although not statistically significant, the p value of the APO E coefficient for oxLDL response was 0.096, indicating that patients who carry the TT allele of the APO E gene tend to present lower plasma oxLDL concentrations. Therefore, the differences in oxidative stress levels observed in this study could not be attributed to diet or to the variant alleles of PON-1, CETP, HMGCR or APO E. This data supports the influence of γ-tocopherol and δ-tocopherol on antioxidant activity, and highlights the need for further studies investigating APO E alleles and LDL oxidation. (AU)

• FAPESP's process: 08/00482-7 - Application of multivariate statistical techniques to evaluate the association between biomarkers of the oxidative stress and dietetic factors in dislipidemic individuals
• Grantee: Inar Castro Erger
• Support Opportunities: Regular Research Grants
• FAPESP's process: 08/10826-5 - Application of multivariate statistical techniques to evaluate the association between biomarkers of the oxidative stress and dietetic factors in dislipidemic individuals
• Grantee: Cyntia Okoshi Fioratti
• Support Opportunities: Scholarships in Brazil - Scientific Initiation