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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Investigation of the Photodynamic Effects of Curcumin Against Candida albicans

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Dovigo, Livia N. [1] ; Pavarina, Ana Claudia [1] ; Ribeiro, Ana Paula D. [1] ; Brunetti, Iguatemy L. [2] ; Costa, Carlos Alberto de S. [1] ; Jacomassi, Denis P. [3] ; Bagnato, Vanderlei S. [3] ; Kurachi, Cristina [3]
Total Authors: 8
[1] UNESP Univ Estadual Paulista, Araraquara Dent Sch, Araraquara, SP - Brazil
[2] UNESP Univ Estadual Paulista, Dept Clin Anal, Araraquara, SP - Brazil
[3] Univ Sao Paulo, Phys Inst Sao Carlos, Sao Carlos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Photochemistry and Photobiology; v. 87, n. 4, p. 895-903, JUL-AUG 2011.
Web of Science Citations: 102

This study describes the association of curcumin with light emitting diode (LED) for the inactivation of Candida albicans. Suspensions of Candida were treated with nine curcumin concentrations and exposed to LED at different fluences. The protocol that showed the best outcomes for Candida inactivation was selected to evaluate the effect of the preirradiation time (PIT) on photodynamic therapy (PDT) effectiveness, the uptake of curcumin by C. albicans cells and the possible involvement of singlet oxygen in the photodynamic action. Curcumin-mediated PDT was also assessed against biofilms. In addition to the microbiological experiments, similar protocols were tested on a macrophage cell line and the effect was evaluated by Methyltetrazolium assay (MTT) and SEM analysis. The optical properties of curcumin were investigated as a function of illumination fluence. When compared with the control group, a statistically significant reduction in C. albicans viability was observed after PDT (P < 0.05), for both planktonic and biofilm cultures. Photodynamic effect was greatly increased with the presence of curcumin in the surrounding media and the PIT of 20 min improved PDT effectiveness against biofilms. Although PDT was phototoxic to macrophages, the therapy was more effective in inactivating the yeast cell than the defense cell. The spectral changes showed a high photobleaching rate of curcumin. (AU)

FAPESP's process: 08/00601-6 - Study of the effectiveness of antimicrobial photodynamic therapy on Candida inactivation and the intracelular damage caused in yeast cells. In vitro and in vivo evaluation of two photosensitizers.
Grantee:Lívia Nordi Dovigo
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 98/14270-8 - Center for Optical and Photonics Science
Grantee:Vanderlei Salvador Bagnato
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 08/03994-9 - Study of the effectiveness of antimicrobial photodynamic therapy on Candida inactivation and the intracelular damage caused in yeast cells: in vitro and in vivo evaluation of two photosensitizers
Grantee:Ana Cláudia Pavarina
Support Opportunities: Regular Research Grants