| Full text | |
| Author(s): |
Clemente-Carvalho, R. B. G.
[1]
;
Alves, A. C. R.
[2]
;
Perez, S. I.
[3]
;
Haddad, C. F. B.
[2]
;
dos Reis, S. F.
[4]
Total Authors: 5
|
| Affiliation: | [1] Univ Estadual Campinas, Programa Posgrad Genet, BR-13083970 Campinas, SP - Brazil
[2] Univ Estadual Paulista, Dept Zool, BR-13506900 Sao Paulo - Brazil
[3] Univ Nacl La Plata, CONICET, Div Antropol, Museo La Plata, RA-1900 La Plata - Argentina
[4] Univ Estadual Campinas, Dept Biol Anim, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Journal of Herpetology; v. 45, n. 1, p. 94-99, MAR 2011. |
| Web of Science Citations: | 5 |
| Abstract | |
A recent study demonstrated marked variation in cranial shape between a population of Brachycephalus ephippium from Jundiai in Sao Paulo state and populations from Atibaia and Sao Francisco Xavier (Sao Paulo) and Macae de Cima in the state of Rio de Janeiro in southeastern Brazil. This result contrasts with earlier work describing differences in cranial shape between the population from Rio de Janeiro and those from Sao Paulo. Here, we investigate the nature and extent of variation between populations of B. ephippium using two lines of evidence. First, we reevaluate patterns of morphological variation by incorporating semi-landmarks into the quantitative description of cranial shape and by using scanning electron microscopy (SEM) to describe qualitative skeletal features. Second, we assess molecular variation in the mitochondrial genes cytochrome b, 12S rRNA, and 16S rRNA, and the nuclear Rag-1 gene, totaling 3,697 base pairs. Geometric analyses of cranial shape and SEM images of skeletal traits show that the population from Macae de Cima and the populations from Sao Paulo differ markedly in morphology and diverge in the cytochrome b, 12S, and 16S rRNA genes by 9.7%, 2.8%, and 4.4%, respectively. Divergence in the nuclear gene Rag-1 is, as expected, much lower (0.6%). These results clearly demonstrate heterogeneity between populations that are all currently diagnosed as B. ephippium and point to the need for further research to ascertain the true diversity currently hidden under the name of B. ephippium. (AU) | |
| FAPESP's process: | 03/12396-4 - Systematic taxonomy and evolution of Brachycephalus (Amphibia: Anura: Brachycephalidae) |
| Grantee: | Ana Claudia Reis Alves |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 08/50928-1 - Speciation of frogs in high-altitude environments |
| Grantee: | Célio Fernando Baptista Haddad |
| Support Opportunities: | Research Projects - Thematic Grants |