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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A systematic study of transfection efficiency and cytotoxicity in HeLa cells using iron oxide nanoparticles prepared with organic and inorganic bases

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Author(s):
Calmon, Marilia Freitas [1] ; de Souza, Aryane Tofanello [2] ; Candido, Natalia Maria [1] ; Bartolomeu Raposo, Maria Irene [3] ; Taboga, Sebastiao [1] ; Rahal, Paula [1] ; Nery, Jose G. [2]
Total Authors: 7
Affiliation:
[1] Sao Paulo State Univ, Dept Biol, Sao Paulo - Brazil
[2] Sao Paulo State Univ, Dept Phys, Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Geosci, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 100, p. 177-184, DEC 1 2012.
Web of Science Citations: 21
Abstract

Magnetic iron oxide nanoparticles (magnetite) (MNPs) were prepared using different organic and inorganic bases. Strong inorganic base (KOH) and organic bases (NH4OH and 1,4-diazabicyclo{[}2.2.2]octane (DABCO)) were used in the syntheses of the MNPs. The MNPs were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM). Fourier transform infrared spectroscopy (FT-IR) and magnetization measurements. MNPs prepared with strong inorganic base yielded an average size of 100 nm, whereas the average size of the MNPs prepared with the organic bases was 150 nm. The main competitive phase for MNPs prepared with the strong inorganic and organic bases was maghemite; however, syntheses with KOH yielded a pure magnetite phase. The transfection study performed with the MNPs revealed that the highest transfection rate was obtained with the MNPs prepared with KOH (74%). The correlation between the magnetic parameters and the transfection ratio without transfection agents indicated that MNPs prepared with KOH were a better vector for possible applications of these MNPs in biomedicine. HeLa cells incubated with MNP-KOH at 10 mu g/mL for 24 and 48 h exhibited a decrease in population in comparison with the control cells and it was presumably related to the toxicity of the MNPs. However, the cells incubated with MNP-KOH at 50 and 100 mu g/mL presented a very small difference in the viability between the cell populations studied at 24 and 48 h. These data illustrate the viability of HeLa cells treated with MNP-KOH and suggest the potential use of these MNPs in biomedical applications. (C) 2012 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 09/11203-4 - Functional analysis of genes involved in carcinogenesis and development of penile cancer
Grantee:Marilia de Freitas Calmon
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 05/54703-6 - Synthesis of enantioselective solid catalysts: the intellectual, technological and synthetic challenge of chiral zeolites
Grantee:Jose Geraldo Nery
Support Opportunities: Research Grants - Young Investigators Grants