A new HPLC-DAD-MS/MS method for the simultaneous d... - BV FAPESP
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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A new HPLC-DAD-MS/MS method for the simultaneous determination of major compounds in the crude extract of Lychnophora salicifolia Mart. (Brazilian arnicao) leaves: Application to chemical variability evaluation

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Author(s):
Gouvea, Dayana Rubio [1] ; Meloni, Fernando [2] ; Bello Ribeiro, Arthur de Barros [1] ; Callegari Lopes, Joao Luis [1] ; Lopes, Norberto Peporine [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, NPPNS, Dept Fis & Quim, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Dept Biol, Lab Ecol Quim & Restauracao Florestal, Fac Ciencias & Letras Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Analytica Chimica Acta; v. 748, p. 28-36, OCT 20 2012.
Web of Science Citations: 13
Abstract

Lychnophora salicifolia Mart., which occurs in the Brazilian Cerrado in the states of Bahia and Minas Gerais as well as in the southeast of the state of Goias, is the most widely distributed and also the most polymorphic species of the genus. This plant is popularly known to have anti-inflammatory and analgesic activities. In this work, we have studied the variation in terms of polar metabolites of ninety-three Lychnophora salicifolia Mart, specimens collected from different regions of the Brazilian Cerrado. Identification of the constituents of this mixture was carried out by analysis of the UV spectra and MS data after chromatographic separation. Twenty substances were identified, including chlorogenic acid derivatives, a flavonoid C-glucoside, and other sesquiterpenes. The analytical method was validated, and the reliability and credibility of the results was ensured for the purposes of this study. The concentration range required for analysis of content variability within the analyzed group of specimens was covered with appropriate values of limits of detection and quantitation, as well as satisfactory precision and recovery. A quantitative variability was observed among specimens collected from the same location, but on average they were similar from a chemical viewpoint. In relation to the study involving specimens from different locations, there were both qualitative and quantitative differences among plants collected from different regions of Brazil. Statistical analysis revealed that there is a correlation between geographical localization and polar metabolites profile for specimens collected from different locations. This is evidence that the pattern of metabolites concentration depends on the geographical distribution of the specimens. (C) 2012 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 08/02323-3 - Interpopulational variation of secondary metabolites of arnicão (Lychnophora salicifolia Mart., Vernonieae, Asteraceae)
Grantee:Dayana Rubio Gouvea
Support Opportunities: Scholarships in Brazil - Master