| Full text | |
| Author(s): |
Bigarella, Carolina L.
[1]
;
Vieira Ferro, Karla P.
[1]
;
Barcellos, Karin S. A.
[1]
;
Martins-de-Souza, Daniel
[2, 3]
;
Traina, Fabiola
[1]
;
Novello, Jose C.
[4]
;
Olalla Saad, Sara T.
[1]
;
Archangelo, Leticia Froehlich
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Estadual Campinas, Hematol & Hemotherapy Ctr, BR-13083878 Campinas, SP - Brazil
[2] Max Planck Inst Psychiat, D-80804 Munich - Germany
[3] Univ Sao Paulo, Fac Med, Lab Neurociencias LIM 27, Inst Psiquiatria, BR-05508 Sao Paulo - Brazil
[4] Univ Estadual Campinas, Inst Biol, Genom & Prote Lab, Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | FEBS Letters; v. 586, n. 19, p. 3522-3528, SEP 21 2012. |
| Web of Science Citations: | 6 |
| Abstract | |
ARHGAP21 is a 217 kDa RhoGAP protein shown to modulate cell migration through the control of Cdc42 and FAK activities. In the present work a 250 kDa-ARHGAP21 was identified by mass spectrometry. This modified form is differentially expressed among cell lines and human primary cells. Co-immunoprecipitations and in vitro SUMOylation confirmed ARHGAP21 specific modification by SUMO2/3 and mapped the SUMOylation site to ARHGAP21 lysine K1443. Immunofluorescence staining revealed that ARHGAP21 co-localizes with SUMO2/3 in the cytoplasm and membrane compartments. Interestingly, our results suggest that ARHGAP21 SUMOylation may be related to cell proliferation. Therefore, SUMOylation of ARHGAP21 may represent a way of guiding its function. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 07/08019-1 - Functional characterization of the CATS protein and its role in cellular proliferation and leukemogenesis |
| Grantee: | Leticia Fröhlich Archangelo |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 07/54870-5 - Functional characterization of the protein and its role in cellular proliferation and leukemogenesis |
| Grantee: | Sara Teresinha Olalla Saad |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 09/07322-8 - ARHGAP21 in SDF-1/CXCR4 signaling and its relation to inefficient hematopoiesis in myelodysplasic syndromes |
| Grantee: | Carolina Louzão Bigarella |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |