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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Post-translational modification of the RhoGTPase activating protein 21, ARHGAP21, by SUMO2/3

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Author(s):
Bigarella, Carolina L. [1] ; Vieira Ferro, Karla P. [1] ; Barcellos, Karin S. A. [1] ; Martins-de-Souza, Daniel [2, 3] ; Traina, Fabiola [1] ; Novello, Jose C. [4] ; Olalla Saad, Sara T. [1] ; Archangelo, Leticia Froehlich [1]
Total Authors: 8
Affiliation:
[1] Univ Estadual Campinas, Hematol & Hemotherapy Ctr, BR-13083878 Campinas, SP - Brazil
[2] Max Planck Inst Psychiat, D-80804 Munich - Germany
[3] Univ Sao Paulo, Fac Med, Lab Neurociencias LIM 27, Inst Psiquiatria, BR-05508 Sao Paulo - Brazil
[4] Univ Estadual Campinas, Inst Biol, Genom & Prote Lab, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: FEBS Letters; v. 586, n. 19, p. 3522-3528, SEP 21 2012.
Web of Science Citations: 6
Abstract

ARHGAP21 is a 217 kDa RhoGAP protein shown to modulate cell migration through the control of Cdc42 and FAK activities. In the present work a 250 kDa-ARHGAP21 was identified by mass spectrometry. This modified form is differentially expressed among cell lines and human primary cells. Co-immunoprecipitations and in vitro SUMOylation confirmed ARHGAP21 specific modification by SUMO2/3 and mapped the SUMOylation site to ARHGAP21 lysine K1443. Immunofluorescence staining revealed that ARHGAP21 co-localizes with SUMO2/3 in the cytoplasm and membrane compartments. Interestingly, our results suggest that ARHGAP21 SUMOylation may be related to cell proliferation. Therefore, SUMOylation of ARHGAP21 may represent a way of guiding its function. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 07/54870-5 - Functional characterization of the protein and its role in cellular proliferation and leukemogenesis
Grantee:Sara Teresinha Olalla Saad
Support type: Regular Research Grants
FAPESP's process: 07/08019-1 - Functional characterization of the CATS protein and its role in cellular proliferation and leukemogenesis
Grantee:Leticia Fröhlich Archangelo
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 09/07322-8 - ARHGAP21 in SDF-1/CXCR4 signaling and its relation to inefficient hematopoiesis in myelodysplasic syndromes
Grantee:Carolina Louzão Bigarella
Support type: Scholarships in Brazil - Post-Doctorate