| Full text | |
| Author(s): |
Innocenti Giorgi, Vanessa S.
[1]
;
Peracoli, Maria Terezinha S.
[1]
;
Peracoli, Jose C.
[2]
;
Witkin, Steven S.
[2, 3]
;
Bannwart-Castro, Camila F.
[1]
Total Authors: 5
|
| Affiliation: | [1] Sao Paulo State Univ, Inst Biosci, Dept Microbiol & Immunol, Botucatu, SP - Brazil
[2] Sao Paulo State Univ, Dept Gynecol & Obstet, Botucatu, SP - Brazil
[3] Cornell Univ, Dept Obstet & Gynecol, Weill Med Coll, New York, NY 10021 - USA
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | JOURNAL OF REPRODUCTIVE IMMUNOLOGY; v. 95, n. 1-2, p. 67-72, SEP 2012. |
| Web of Science Citations: | 35 |
| Abstract | |
Preeclampsia (PE) is a complication of human pregnancy associated with an intense inflammatory response involving leukocyte activation, as well as elevated production of pro-inflammatory cytokines. The nuclear transcription factor-kappa B (NF-kappa B) is present in cells of the immune system and is responsible for transcription of genes coding for pro-inflammatory proteins. Silibinin is the main component of silymarin, a polyphenolic extract obtained from fruits and seeds of Silybum marianum with potent hepatoprotective and anti-inflammatory activities. In this study, we assessed whether silibinin modulated NF-kappa B activity and the production of pro-inflammatory cytokines by peripheral blood mononuclear cells (PBMCs) from preeclamptic patients. PBMC from women with PE, normotensive (NT) pregnant women, and nonpregnant (NP) women were cultured with or without silibinin (5 mu M and 50 mu M) and 1 mu g/mL lipopolysaccharide (LPS) for 18 h. The supernatants were assayed for tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) by ELISA. Cells were cultured for 30 min to evaluate NF-kappa B activity. There was increased endogenous activation of NF-kappa B as well as TNF-alpha and IL-1 beta release by PBMC in the PE group compared with the NT and NP groups. A positive correlation between NF-kappa B activity and cytokine production was also observed in the PE group. Silibinin was capable of reducing, at least in part, the levels of NF-kappa B and cytokines TNF-alpha and IL-1 beta in preeclamptic women. We conclude that silibinin exhibits potent anti-inflammatory activity on PBMC from preeclamptic women by downmodulation of NF-kappa B activation and inflammatory cytokine production. (C) 2012 Elsevier Ireland Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 10/00776-0 - Effect of silibinin on nuclear factor kappa-B and inflammatory cytokines production by mononuclear cells of pregnant women with preeclampsia |
| Grantee: | Vanessa Silvestre Innocenti Giorgi |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |