Voltammetric detection of the interactions between RNO2 circle- and electron acceptors in aqueous medium at highly boron doped diamond electrode (HBDDE)

Author(s)

Julião, Murilo S. da S. ; Ferreira, Elizabeth I. ^[2] ; Ferreira, Neidenêi G. ; Serrano, Silvia H. P.

• Document type: Journal article
• Source: Electrochimica Acta; v. 51, n. 24, p. 5080-5086, July 2006.
• Field of knowledge: Health Sciences - Pharmacy

Abstract

This paper describes the electrochemical behavior of the nitrofurazone (NFZ), in predominantly aqueous medium, in the absence and presence of glutathione (reduced form) (GSH), L-Cysteine (Cys) and O-2 using a highly boron doped diamond electrode (HBDDE). In presence of [Thiol] >= 3.7 x 10(-2) mol L-1 NFZ is directly reduced to RNO-Thiol adducts in an electrochemical process involving two electrons and two protons. On the other side, 02 acts as a RNO2 center dot- scavenger and the velocity constant for the reaction, kO(2), is 60 L mol(-1) s(-1). The process is catalytic and can be used to the analytical determination of NFZ in the range of 9.9 x 10(-7) <= [NFZ] <= 1.1 x 10(-5) mol L-1 at pH 8.0, with sensitivity of 2.2 x 10(6) mu A mol(-1) cm(-2) and detection limit of 3.4 x 10(-7) mol L-1. The analytical parameters were similar to those obtained at pH 4.0 using the direct reduction of NFZ to the respective amine derivative in a process involving six electrons and six protons. The characterization of NFZ global reduction process in aqueous medium and at relative low scan rate, 100 mV s(-1), was only possible due the intrinsic superficial characteristics of the HBDDE, which stabilize the RNO2 center dot- free radical, allowing to work in a large potential window, without losing the RNO2 center dot- oxidation signal. (AU)

• FAPESP's process: 01/01192-3 - Potential antitripanosomal derived from nitro-heterocyclic compounds
• Grantee: Elizabeth Igne Ferreira
• Support Opportunities: Research Projects - Thematic Grants