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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Development of an enzymeless electroanalytical method for the indirect detection of creatinine in urine samples

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de Araujo, William R. [1] ; Salles, Maiara O. [1] ; Paixao, Thiago R. L. C. [1]
Total Authors: 3
[1] Univ Sao Paulo, Inst Quim, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: SENSORS AND ACTUATORS B-CHEMICAL; v. 173, p. 847-851, OCT 2012.
Web of Science Citations: 24

The aim of this study is to develop a new enzymeless electroanalytical method for the indirect quantification of creatinine from urine sample. This method is based on the electrochemical monitoring of picrate anion reduction at a glassy carbon electrode in an alkaline medium before and after it has reacted with creatinine (Jaffe's reaction). By using the differential pulse voltammetry technique under the optimum experimental conditions (step potential, amplitude potential, reaction time, and temperature), a linear analytical curve was obtained for concentrations of creatinine ranging from 1 to 80 mu mol L-1, with a detection limit of 380 nmol L-1. This proposed method was used to measure creatinine in human urine without the interference of most common organic species normally present in biological fluids (e.g., uric acid, ascorbic acid, glucose, and phosphocreatinine). The results obtained using urine samples were highly similar to the results obtained using the reference spectrophotometric method (at a 95% confidence level). (C) 2012 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 09/07859-1 - Development of voltammetric electronic tongues towards the characterization and identification of adulteration and falsification processes of biofuels
Grantee:Thiago Regis Longo Cesar da Paixão
Support type: Regular Research Grants
FAPESP's process: 11/19903-5 - Development of electroanalytical methods for detection/discrimination of drugs of abuse, its adulterants and explosives in forensic samples
Grantee:William Reis de Araujo
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 11/11115-8 - Development of a hibrid tongue (electrochemistry and colorimetric) aiming the discrimination of forensic samples.
Grantee:Maiara Oliveira Salles
Support type: Scholarships in Brazil - Post-Doctorate