| Full text | |
| Author(s): |
Alexanda Dias Reis
;
Maria Cristina Domingues Fink
;
Clarisse Martins Machado
;
José de Paula Paz Jr.
;
Renato Reis Oliveira
;
Adriana Fumie Tateno
;
Adriana Freire Machado
;
Maria Regina Cardoso
[8]
;
Claudio Sérgio Pannuti
Total Authors: 9
|
| Document type: | Journal article |
| Source: | Revista do Instituto de Medicina Tropical de São Paulo; v. 50, n. 1, p. 37-40, 2008-02-00. |
| Abstract | |
A total of 316 samples of nasopharyngeal aspirate from infants up to two years of age with acute respiratory-tract illnesses were processed for detection of respiratory syncytial virus (RSV) using three different techniques: viral isolation, direct immunofluorescence, and PCR. Of the samples, 36 (11.4%) were positive for RSV, considering the three techniques. PCR was the most sensitive technique, providing positive findings in 35/316 (11.1%) of the samples, followed by direct immunofluorescence (25/316, 7.9%) and viral isolation (20/315, 6.3%) (p < 0.001). A sample was positive by immunofluorescence and negative by PCR, and 11 (31.4%) were positive only by RT-PCR. We conclude that RT-PCR is more sensitive than IF and viral isolation to detect RSV in nasopharyngeal aspirate specimens in newborn and infants. (AU) | |