Effect of angiotensin II and losartan on the phagocytic activity of peritoneal macrophages from Balb/C mice

Author(s)

Paula Belline ^[1] ; Patrícia da Silva Melo ^[2] ; Marcela Haun ^[3] ; Fernanda Boucault Palhares ^[4] ; Patrícia Aline Boer ^[5] ; José Antônio Rocha Gontijo ^[6] ; José Francisco Figueiredo ^[7]

Affiliation

^[1] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Clínica Médica

^[2] Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Bioquímica - Brasil

^[3] Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Bioquímica - Brasil

^[4] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Clínica Médica

^[5] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Clínica Médica

^[6] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Clínica Médica

^[7] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Clínica Médica

• Document type: Journal article
• Source: Memórias do Instituto Oswaldo Cruz; v. 99, n. 2, p. 167-172, 2004-03-00.

Abstract

Angiotensin II (AII), a product of rennin-angiotensin system, exerts an important role on the function of immune system cells. In this study, the effect of AII on the phagocytic activity of mouse peritoneal macrophages was assessed. Mice peritoneal macrophages were cultured for 48 h and the influence of different concentrations of AII (10-14 to 10-7 M) and/or losartan, 10-16 to 10-6 M), an AT1 angiotensin receptor antagonist, on phagocytic activity and superoxide anion production was determined. Dimethylthiazoldiphenyltetrazolium bromide reduction and the nucleic acid content were used to assess the cytotoxicity of losartan. A stimulatory effect on phagocytic activity (P < 0.05) was observed with 10-13 M and 10-12 M AII concentrations. The addition of losartan (up to10-14 M) to the cell cultures blocked (P < 0.001) the phagocytosis indicating the involvement of AT1 receptors. In contrast, superoxide anion production was not affected by AII or losartan. The existence of AT1 and AT2 receptors in peritoneal macrophages was demonstrated by immunofluorescence microscopy. These results support the hypothesis that AII receptors can modulate murine macrophage activity and phagocytosis, and suggest that AII may have a therapeutic role as an immunomodulatory agent in modifying the host resistance to infection. (AU)

• FAPESP's process: 00/12216-8 - Cloning and characterization of novel human genes related to spectrin, ankyrin and anion exchangers
• Grantee: Sara Teresinha Olalla Saad
• Support Opportunities: Research Projects - Thematic Grants