Effect of photobiomodulation on expression of IL-1 beta in skeletal muscle following acute injury

Author(s)

Santos Fernandes, Kristianne Porta ^{[1, 2]} ; Alves, Agnelo Neves ^[1] ; Nunes, Fabio Daumas ^[3] ; Costa Souza, Nadhia Helena ^[1] ; Silva, Jr., Jose Antonio ^[1] ; Bussadori, Sandra Kalil ^[1] ; Mesquita Ferrari, Raquel Agnelli ^[1]

Affiliation

^[1] Univ Nove de Julho UNINOVE, BR-05001100 Sao Paulo - Brazil

^[2] Univ Nove de Julho UNINOVE, Dept Pos Grad, BR-05001100 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Dent, Dept Oral Pathol, Sao Paulo - Brazil

• Document type: Journal article
• Source: Lasers in Medical Science; v. 28, n. 3, p. 1043-1046, MAY 2013.
• Web of Science Citations: 36

Abstract

Muscle repair is regulated by growth factors and cytokines. Low-level laser therapy (LLLT) seems to influence acute inflammation and accelerate skeletal muscle repair. This study verifies the effect of LLLT on the expression of IL-1 beta in the tibialis anterior (TA) muscle of rats following acute injury. Wistar rats (n = 35) were allocated into three groups: control (without lesion and LLLT, n = 5), injury group (n = 15), and injury + LLLT group (n = 15). The acute injury was induced by the contact with a cooled metal probe (3 mm in diameter) during 10 s, twice, in the same muscle area. LLLT was used three times a week using the InGaAlP laser (660 nm; beam spot of 0.04 cm(2), output power of 20 mW, power density of 500 mW/cm(2), and energy density of 5 J/cm(2) during 10 s). The animals were analyzed at 1, 7, and 14 days following injury. TA muscles samples were used for obtaining total RNA and performing cDNA synthesis. Real-time polymerase chain reactions were realized using IL-1 beta primer. There was a decrease in IL-1 beta expression after 7 days in LLLT group in comparison with the no treated group. In conclusion, LLLT was able to decrease IL-1 beta expression during the skeletal muscle repair following an acute injury. (AU)

• FAPESP's process: 11/04452-8 - Photobiomodulation on regulatory myogenic factors expression during skeletal muscle repair following cryolesion in rats
• Grantee: Agnelo Neves Alves
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 11/17638-2 - Effects of low level laser on regulatory myogenic factors expression and matrix metalloprotease 2 activity during skeletal muscle repair following cryolesion in rats
• Grantee: Raquel Agnelli Mesquita Ferrari
• Support Opportunities: Regular Research Grants