| Full text | |
| Author(s): |
Vanessa Fátima de Oliveira
[1]
;
Nair Massumi Itaya
[2]
;
Eny Iochevet Segal Floh
[3]
;
Maria Angela Machado de Carvalho
[4]
Total Authors: 4
|
| Affiliation: | [1] Instituto de Botânica - Brasil
[2] Universidade do Grande ABC - Brasil
[3] Universidade de São Paulo. Instituto de Biociências - Brasil
[4] Instituto de Botânica - Brasil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Hoehnea; v. 36, n. 1, p. 89-97, 2009-03-00. |
| Abstract | |
Smallanthus sonchifolius is an Asteraceae native from the Andes Mountains that accumulates inulin-type fructans in the underground reserve organs, the tuberous roots and the rhizophores. Yellow callus was obtained from tuberous roots, giving origin, spontaneously to red callus. Fructans and their metabolizing enzymes were analyzed in calli of both types, showing similar fructan contents in both lineages. However, synthesizing activity was higher in red callus, while hydrolyzing activity was higher in yellow callus. HPAEC/ PAD analyses revealed the presence of fructo-oligosaccharides in both calli with degrees of polymerization lower than those found in plants cultured ex vitro, indicating that this characteristic was maintained in undifferentiated cells. The presence of fructans and the differences between the activities of fructosyltransferases and hydrolases found in red and yellow calli indicate this material as suitable for studies of fructan metabolism under controlled conditions. (AU) | |
| FAPESP's process: | 98/05124-8 - Conservation and sustainable use of the plant biodiversity from the cerrado and Atlantic Forest: the storage of carbohydrates and their role on the adaptation and maintainance of plants in their natural environment |
| Grantee: | Marcos Silveira Buckeridge |
| Support Opportunities: | BIOTA-FAPESP Program - Thematic Grants |
| FAPESP's process: | 05/04139-7 - Carbohydrates of tropical species as modulators of ecophysiological processes and as environmental stress response markers |
| Grantee: | Marcia Regina Braga |
| Support Opportunities: | Research Projects - Thematic Grants |