Advanced search
Start date
Betweenand
(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A new rapid and sensitive enzymatic method for extraction and quantification of starch in plant material

Full text
Author(s):
Lourdes Isabel Velho do Amaral [1] ; Marília Gaspar [2] ; Paula Moreira Felix Costa [3] ; Marcos Pereira Marinho Aidar [4] ; Marcos Silveira Buckeridge [5]
Total Authors: 5
Affiliation:
[1] Universidade Federal de Brasília. Departamento de Botânica - Brasil
[2] Instituto de Botânica. Seção de Fisiologia e Bioquímica de Plantas - Brasil
[3] Instituto de Botânica. Seção de Fisiologia e Bioquímica de Plantas - Brasil
[4] Instituto de Botânica. Seção de Fisiologia e Bioquímica de Plantas - Brasil
[5] Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica - Brasil
Total Affiliations: 5
Document type: Journal article
Source: Hoehnea; v. 34, n. 4, p. 425-431, 2007-00-00.
Abstract

In this work we compare methods normally used for starch determination in plant materials. The comparison between chemical (McCready's method) and an enzymatic method proposed here showed that although McCready's method is appropriate for most plant materials, in certain cases where cell wall polysaccharides (pectins and hemicelluloses) are present, the results may be significantly altered. However, using the enzymatic method described here afforded accurate estimation of starch content in such tissues. The enzymatic method proposed in this work is an affordable option for precise determination of starch contents in several plant tissues. (AU)

FAPESP's process: 98/05124-8 - Conservation and sustainable use of the plant biodiversity from the cerrado and Atlantic Forest: the storage of carbohydrates and their role on the adaptation and maintainance of plants in their natural environment
Grantee:Marcos Silveira Buckeridge
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants