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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of Fluoride-Treated Enamel on Indirect Cytotoxicity of a 16% Carbamide Peroxide Bleaching Gel to Pulp Cells

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Author(s):
Diana Gabriela Soares [1] ; Ana Paula Dias Ribeiro [2] ; Adriano Fonseca Lima [3] ; Nancy Tomoko Sacono [4] ; Josimeri Hebling [5] ; Carlos Alberto de Souza Costa [6]
Total Authors: 6
Affiliation:
[1] UNESP - Univ Estadual Paulista. Araraquara Dental School. Department of Dental Materials and Prosthodontics - Brasil
[2] UnB - Federal University of Brasília. Department of Dentistry - Brasil
[3] UNICAMP - University of Campinas. Piracicaba Dental School. Department of Restorative Dentistry - Brasil
[4] UFG - Federal University of Goiás. Department of Dentistry - Brasil
[5] UNESP - Univ Estadual Paulista. Araraquara Dental School. Department of Orthodontics and Pediatric Dentistry - Brasil
[6] UNESP - Univ Estadual Paulista. Araraquara Dental School. Department of Physiology and Pathology - Brasil
Total Affiliations: 6
Document type: Journal article
Source: Brazilian Dental Journal; v. 24, n. 2, p. 121-127, 2013-04-00.
Abstract

The aim of this study was to evaluate the possibility of fluoride solutions applied to enamel to protect pulp cells against the trans-enamel and transdentinal cytotoxicity of a 16% carbamide peroxide (CP) bleaching gel. The CP gel was applied to enamel/dentin discs adapted to aicial pulp chambers (8 h/day) during 1, 7 or 14 days, followed by fluoride (0.05% or 0.2%) application for 1 min. The extracts (culture medium in contact with dentin) were applied to MDPC-23 cells for 1 h, and cell metabolism (MTT assay), alkaline phosphatase (ALP) activity and cell membrane damage (flow cytometry) were analyzed. Knoop microhardness of enamel was also evaluated. Data were analyzed statistically by ANOVA and Kruskal-Wallis tests (α=0.05). For the MTT assay and ALP activity, significant reductions between the control and the bleached groups were observed (p<0.05). No statistically significant difference occurred among bleached groups (p>0.05), regardless of fluoride application or treatment days. Flow cytometry analysis demonstrated 30% of cell membrane damage in all bleached groups. After 14 days of treatment, the fluoride-treated enamel presented significantly higher microhardness values than the bleached-only group (p<0.05). It was concluded that, regardless of the increase in enamel hardness due to the application of fluoride solutions, the treated enamel surface did not prevent the toxic effects caused by the 16% CP gel to odontoblast-like cells. (AU)

FAPESP's process: 11/09385-7 - New parameters for tooth bleaching: an evaluation of efficacy, citotoxicity and molecular effects
Grantee:Diana Gabriela Soares dos Passos
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 11/12938-8 - New parameters for tooth bleaching: an evaluation of efficacy, citotoxicity and molecular effects
Grantee:Carlos Alberto de Souza Costa
Support Opportunities: Regular Research Grants