A multi-purpose flow manifold for the spectrophotometric determination of sulphide, sulphite and ethanol involving gas diffusion: Application to wine and molasses analysis

Author(s)

Silva, Claudineia R. ^[1] ; Gomes, Taciana F. ^[1] ; Barros, Valdemir A. F. ^[1] ; Zagatto, Elias A. G. ^[1]

Affiliation

^[1] Univ Sao Paulo, Ctr Nucl Energy Agr, Res Support Ctr Technol & Innovat Sustainable Agr, BR-13400970 Piracicaba, SP - Brazil

• Document type: Journal article
• Source: Talanta; v. 113, p. 118-122, SEP 15 2013.
• Web of Science Citations: 7

Abstract

A simple and rugged flow set up was designed for spectrophotometric determination of sulphide, sulphite and ethanol aiming at quality assessment of wines, control of industrial fermentation, and selection of yeast strain. The different assays involved gas diffusion through a Teflon planar membrane and were carried out after minor modifications in the manifold, namely reagent composition and total flow rate. Main figures of merit: linear analytical curves=0.50-6.0 mgL(-1)S(2-), 2.5-20.0 mg L-1 SO3- and 5.0-25.0% (v/v) of ethanol; detection limits (3 sigma)=0.035 mg L-1 S2-, 0.2 mg L-1 SO3- and 0.18% (v/v) of ethanol; peak height r.s.d.=2.18% for 4.03 mg L-1 S2- spiked molasses, 2.21% for a 9.82 mg L-1 SO3- wine and 2.07% for a typical wine (12.53% v/v of ethanol), sampling rate=15, 57 and 29 h(-1), reagent consumptions=1.9 mu mol of N,N-dimethyl-p-phenylenediamine, 1.68 mu g of Malachite green and 0.68 mmol Cr(VI) per determination, respectively. (C) 2013 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 10/00972-4 - Exploitation of pulsed flow and gas diffusion in a polyvalent analytical flow system: sulfide, sulfite and ethanol monitoring during alcoholic fermentation.
• Grantee: Claudineia Rodrigues da Silva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 11/23498-9 - Advanced flow systems for technological, agronomical and environmental analysis
• Grantee: Elias Ayres Guidetti Zagatto
• Support Opportunities: Research Projects - Thematic Grants