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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of laser therapy on skeletal muscle repair process in diabetic rats

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Author(s):
Franca, Cristiane Miranda [1] ; Santana, Cristiano de Loura [1] ; Takahashi, Camila Borin [2] ; Alves, Agnelo Neves [1] ; De Souza Mernick, Ana Paula [2] ; Santos Fernandes, Kristianne Porta [1] ; Teixeira da Silva, Daniela de Fatima [1] ; Bussadori, Sandra Kalil [3] ; Mesquita-Ferrari, Raquel Agnelli [1]
Total Authors: 9
Affiliation:
[1] Univ Nove de Julho, Postgrad Program Biophoton Appl Hlth Sci, BR-01504001 Sao Paulo - Brazil
[2] Univ Nove de Julho, Sch Dent, BR-01504001 Sao Paulo - Brazil
[3] Univ Nove de Julho, Postgrad Program Rehabil Sci, BR-01504001 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Lasers in Medical Science; v. 28, n. 5, p. 1331-1338, SEP 2013.
Web of Science Citations: 16
Abstract

Skeletal muscle myopathy is a common source of disability in diabetic patients. This study evaluated whether low-level laser therapy (LLLT) influences the healing morphology of injured skeletal muscle. Sixty-five male Wistar rats were divided as follows: (1) sham; (2) control; (3) diabetic; (4) diabetic sham; (5) nondiabetic cryoinjured submitted to LLLT (LLLT); (6) diabetic cryoinjured submitted to LLLT (D-LLLT); and (7) diabetic cryoinjured non-treated (D). Diabetes was induced with streptozotocin. Anterior tibialis muscle was cryoinjured and received LLLT daily (780 nm, 5 J/cm(2), 10 s per point; 0.2 J; total treatment, 1.6 J). Euthanasia occurred on day 1 in groups 1, 2, 3, and 4 and on days 1, 7, and 14 in groups 5, 6, and 7. Muscle samples were processed for H\&E and Picrosirius Red and photographed. Leukocytes, myonecrosis, fibrosis, and immature fibers were manually quantified using the ImageJ software. On day 1, all cryoinjured groups were in the inflammatory phase. The D group exhibited more myonecrosis than LLLT group (p < 0.05). On day 14, the LLLT group was in the remodeling phase; the D group was still in the proliferative phase, with fibrosis, chronic inflammation, and granulation tissue; and the D-LLLT group was in an intermediary state in relation to the two previous groups. Under polarized light, on day 14, the LLLT and D-LLLT groups had organized collagen bundles in the perimysium, whereas the diabetic groups exhibited fibrosis. LLLT can have a positive effect on the morphology of skeletal muscle during the tissue repair process by enhancing the reorganization of myofibers and the perimysium, reducing fibrosis. (AU)

FAPESP's process: 11/04452-8 - Photobiomodulation on regulatory myogenic factors expression during skeletal muscle repair following cryolesion in rats
Grantee:Agnelo Neves Alves
Support type: Scholarships in Brazil - Master
FAPESP's process: 11/20867-3 - Immunolocalization of skeletal muscle laminin and fibronectin in the healing process of diabetic rats treated with low level lasertherapy
Grantee:Camila Borin Takahashi
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 11/17638-2 - Effects of low level laser on regulatory myogenic factors expression and matrix metalloprotease 2 activity during skeletal muscle repair following cryolesion in rats
Grantee:Raquel Agnelli Mesquita Ferrari
Support type: Regular Research Grants