Lipase Production by Botryosphaeria ribis EC-01 on Soybean and Castorbean Meals: Optimization, Immobilization, and Application for Biodiesel Production

Author(s)

Andrade, Milena M. ^[1] ; Barbosa, Aneli M. ^{[2, 3]} ; Bofinger, Matheus R. ^[1] ; Dekker, Robert F. H. ^[2] ; Messias, Josana M. ^[3] ; Guedes, Carmen L. B. ^[1] ; Zaminelli, Tiago ^[3] ; de Oliveira, Bruno H. ^[4] ; de Lima, Valeria M. G. ^[4] ; Dall'Antonia, Luiz H. ^[1]

Affiliation

^[1] Univ Estadual Londrina, CCE, Dept Quim, BR-86051990 Londrina, Parana - Brazil

^[2] Lakehead Univ, Biorefining Res Inst, Thunder Bay, ON P7B 5E1 - Canada

^[3] Univ Estadual Londrina, CCE, Dept Bioquim & Biotecnol, BR-86051990 Londrina, Parana - Brazil

^[4] Univ Estadual Paulista Julio de Mesquita Filho UN, Dept Ciencias Biol, BR-19806900 Sao Paulo - Brazil

• Document type: Journal article
• Source: Applied Biochemistry and Biotechnology; v. 170, n. 7, p. 1792-1806, AUG 2013.
• Web of Science Citations: 3

Abstract

The effects of soybean and castorbean meals were evaluated separately, and in combinations at different ratios, as substrates for lipase production by Botryosphaeria ribis EC-01 in submerged fermentation using only distilled water. The addition of glycerol analytical grade (AG) and glycerol crude (CG) to soybean and castorbean meals separately and in combination, were also examined for lipase production. Glycerol-AG increased enzyme production, whereas glycerol-CG decreased it. A 2(4) factorial design was developed to determine the best concentrations of soybean meal, castorbean meal, glycerol-AG, and KH2PO4 to optimize lipase production by B. ribis EC-01. Soybean meal and glycerol-AG had a significant effect on lipase production, whereas castorbean meal did not. A second treatment (2(2) factorial design central composite) was developed, and optimal lipase production (4,820 U/g of dry solids content (ds)) was obtained when B. ribis EC-01 was grown on 0.5 % (w/v) soybean meal and 5.2 % (v/v) glycerol in distilled water, which was in agreement with the predicted value (4,892 U/g ds) calculated by the model. The unitary cost of lipase production determined under the optimized conditions developed ranged from US\$0.42 to 0.44 based on nutrient costs. The fungal lipase was immobilized onto Celite and showed high thermal stability and was used for transesterification of soybean oil in methanol (1:3) resulting in 36 % of fatty acyl alkyl ester content. The apparent K (m) and V (max) were determined and were 1.86 mM and 14.29 mu mol min(-1) mg(-1), respectively. (AU)

• FAPESP's process: 10/07998-9 - Transesterification of vegetable oils by immobilized lipase from Fusarium sp. produced by submerged and solid state fermentation
• Grantee: Valéria Marta Gomes de Lima
• Support type: Regular Research Grants