In vitro and in vivo studies of a novel nanohydroxyapatite/superhydrophilic vertically aligned carbon nanotube nanocomposites

Author(s)

Lobo, Anderson Oliveira ^[1] ; Siqueira, Idalia A. W. B. ^[1] ; das Neves, Marcele F. ^[1] ; Marciano, Fernanda Roberta ^[1] ; Corat, Evaldo Jose ^[2] ; Corat, Marcus Alexandre F. ^[3]

Affiliation

^[1] Univ Vale do Paraiba UNIVAP, Lab Biomed Nanotechnol NANOBIO, BR-12224000 Sao Jose Dos Campos, SP - Brazil

^[2] Univ Estadual Campinas UNICAMP, Ctr Multidisciplinar Invest Biol CEMIB, BR-13083877 Campinas, SP - Brazil

^[3] Natl Inst Space Res INPE, Associated Lab Sensors & Mat LAS, BR-12227010 Sao Jose Dos Campos, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE; v. 24, n. 7, p. 1723-1732, JUL 2013.
• Web of Science Citations: 13

Abstract

An association between in vitro and in vivo studies has been demonstrated for the first time, using a novel nanohydroxyapatite/superhydrophilic vertically aligned multiwalled carbon nanotube (nHAp/VAMWCNT-O-2) nanocomposites. Human osteoblast cell culture and bone defects were used to evaluate the in vitro extracellular matrix (ECM) calcification process and bone regeneration, respectively. The in vitro ECM calcification process of nHAp/VAMWCNT-O-2 nanocomposites were investigated using alkaline phosphatase assay. The in vivo biomineralization studies were carried out on bone defects of C57BL/6/JUnib mice. Scanning electron microscopy, micro-energy dispersive spectroscopy, X-ray photoelectron spectroscopy, and X-ray difractometry analyses confirmed the presence of the nHAp crystals. nHAp/VAMWCNT-O-2 nanocomposites induced in vitro calcification of the ECM of human osteoblast cells in culture after only 24 h. Bone regeneration with lamellar bone formation after 9 weeks was found in the in vivo studies. Our findings make these new nanocomposites very attractive for application in bone tissue regeneration. (AU)

• FAPESP's process: 11/20345-7 - Study of nanoparticle-incorporated diamond-like carbon films for biomedical applications
• Grantee: Fernanda Roberta Marciano
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 11/17877-7 - Development of new polymeric scaffolds by electrospinning technique with incorporation of vertically aligned carbon nanotubes and nanohidroxyapatite for bone tissue regeneration
• Grantee: Anderson de Oliveira Lobo
• Support Opportunities: Research Grants - Young Investigators Grants