Isolation by pressurised fluid extraction (PFE) and identification using CPC and HPLC/ESI/MS of phenolic compounds from Brazilian cherry seeds (Eugenia uniflora L.)

Author(s)

Oliveira, Alessandra L. ^[1] ; Destandau, Emilie ^[2] ; Fougere, Laetitia ^[2] ; Lafosse, Michel ^[2]

Affiliation

^[1] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, Dept Engn Alimentos, BR-13635900 Pirassununga, SP - Brazil

^[2] Univ Orleans, CNRS, Inst Chim Organ & Analyt, UMR 6005, F-45067 Orleans - France

• Document type: Journal article
• Source: Food Chemistry; v. 145, p. 522-529, FEB 15 2014.
• Web of Science Citations: 23

Abstract

Brazilian cherry seeds are a waste product from juice and frozen pulp production and, the seeds composition was investigated to valorize this by-product. Compounds separation was performed with ethanol by pressurised fluid extraction (PFE). Here we determine the effect of temperature (7), static time (ST), number of cycles (C), and flush volume (VF) on the yield, composition and total phenolic content (TPC) of the seed extracts. T, ST and their interaction positively influenced yield and TPC. Extracts were fractionated by high performance liquid chromatography (HPLC) and centrifugal partition chromatography (CPC). The collected fractions characterizations were made by electrospray ionisation mass spectrometry (ESI/MS) and high resolution mass spectrometry (HRMS) indicated the presence of ellagic acid pentoside and deoxyhexose, quercitrin and kaempferol pentoside. All of these compounds have antioxidant properties and normally are found in plant extracts. These results confirm that Brazilian cherry seed extract is a potentially valuable source of antioxidants. (C) 2013 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 08/00148-0 - Collection and analysis of extracts from seeds of pitanga (Eugenia uniflora L.) obtained by use of supercritical carbon dioxide
• Grantee: Alessandra Lopes de Oliveira
• Support Opportunities: Regular Research Grants