Changes in the interface of nanoparticles of gold / silver and lipases: their effe...
| Full text | |
| Author(s): |
Kisukuri, Camila M.
[1]
;
Macedo, Alexandra
[2]
;
Oliveira, Caio C. S.
[2]
;
Camargo, Pedro H. C.
[2]
;
Andrade, Leandro H.
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Sao Paulo, Lab Quim Fina & Biocatalise, BR-05508900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Quim, GrAND Grp Avanco Design Nanomat, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Langmuir; v. 29, n. 51, p. 15974-15980, DEC 24 2013. |
| Web of Science Citations: | 7 |
| Abstract | |
We employed thiol-funcionalized AgAu nanoshells (AgAu NSs) as supports for the covalent attachment of lipases (BCL, Burkholderia cepacia lipase; PPL, pancreatic porcine lipase). Specifically, we were interested in investigating the effect of the nature/size of the spacer in AgAu NSs-functionalized organic thiols over the covalent attachment of lipases. The catalytic performance of AgAu-lipase systems was measured in the kinetic resolution of (R,S)-1-(phenyl)ethanol via a transesterification reaction. In comparison to free BCL, the lipase attached to AgAu NSs using a small spacer such as cysteamine or mercaptoacetic acid, with the largest spacer mercaptoundecanoic acid, had the fastest conversion rate. The recycling potential for BCL was investigated. After three reaction cycles, the enzyme activity was kept at around 90% of the initial value. The results described herein show that the size of the spacer plays an important role in optimizing lipase activities in metallic nanoshells as solid supports. (AU) | |
| FAPESP's process: | 12/11201-4 - Changes in the interface of nanoparticles of gold / silver and lipases: their effects on enzyme activity |
| Grantee: | Camila de Menezes Kisukuri |
| Support type: | Scholarships in Brazil - Master |
| FAPESP's process: | 12/14196-1 - Enzymatic reactions: exploiting your potential as synthetic tool |
| Grantee: | Luiz Fernando da Silva Júnior |
| Support type: | Regular Research Grants |
| FAPESP's process: | 11/06847-0 - Multimetallic nanostructures with hollow interiors and well-defined sizes, shapes and compositions |
| Grantee: | Pedro Henrique Cury Camargo |
| Support type: | Regular Research Grants |