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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Investigating the Influence of the Interface in Thiol-Functionalized Silver-Gold Nanoshells over Lipase Activity

Full text
Author(s):
Kisukuri, Camila M. [1] ; Macedo, Alexandra [2] ; Oliveira, Caio C. S. [2] ; Camargo, Pedro H. C. [2] ; Andrade, Leandro H. [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Lab Quim Fina & Biocatalise, BR-05508900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Quim, GrAND Grp Avanco Design Nanomat, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Langmuir; v. 29, n. 51, p. 15974-15980, DEC 24 2013.
Web of Science Citations: 7
Abstract

We employed thiol-funcionalized AgAu nanoshells (AgAu NSs) as supports for the covalent attachment of lipases (BCL, Burkholderia cepacia lipase; PPL, pancreatic porcine lipase). Specifically, we were interested in investigating the effect of the nature/size of the spacer in AgAu NSs-functionalized organic thiols over the covalent attachment of lipases. The catalytic performance of AgAu-lipase systems was measured in the kinetic resolution of (R,S)-1-(phenyl)ethanol via a transesterification reaction. In comparison to free BCL, the lipase attached to AgAu NSs using a small spacer such as cysteamine or mercaptoacetic acid, with the largest spacer mercaptoundecanoic acid, had the fastest conversion rate. The recycling potential for BCL was investigated. After three reaction cycles, the enzyme activity was kept at around 90% of the initial value. The results described herein show that the size of the spacer plays an important role in optimizing lipase activities in metallic nanoshells as solid supports. (AU)

FAPESP's process: 12/11201-4 - Changes in the interface of nanoparticles of gold / silver and lipases: their effects on enzyme activity
Grantee:Camila de Menezes Kisukuri
Support type: Scholarships in Brazil - Master
FAPESP's process: 12/14196-1 - Enzymatic reactions: exploiting your potential as synthetic tool
Grantee:Luiz Fernando da Silva Júnior
Support type: Regular Research Grants
FAPESP's process: 11/06847-0 - Multimetallic nanostructures with hollow interiors and well-defined sizes, shapes and compositions
Grantee:Pedro Henrique Cury Camargo
Support type: Regular Research Grants