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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of Ischemia and Reperfusion on Subpopulations of Rat Enteric Neurons Expressing the P2X7 Receptor

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Palombit, Kelly [1] ; Mendes, Cristina Eusebio [1] ; Tavares-de-Lima, Wothan [2] ; Silveira, Mariana Povoa [1, 3] ; Castelucci, Patricia [1]
Total Authors: 5
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, BR-05508900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, BR-05508900 Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Digestive Diseases and Sciences; v. 58, n. 12, p. 3429-3439, DEC 2013.
Web of Science Citations: 12

Intestinal ischemia followed by reperfusion (I/R) may occur following intestinal obstruction. In rats, I/R in the small intestine leads to structural changes accompanied by neuronal death. To analyze the impact of I/R injury on different neuronal populations in the myenteric plexus of rat ileum. The ileal artery was occluded for 35 min and animals were euthanized 6, 24, and 72 h, and 1 week later. Immunohistochemistry was performed with antibodies against the P2X7 receptor as well as nitric oxide synthase (NOS), calbindin, calretinin, choline acetyltransferase (ChAT), or the pan-neuronal marker anti-HuC/D. Double immunolabeling demonstrated that 100 % of NOS-, calbindin-, calretinin-, and ChAT-immunoreactive neurons in all groups expressed the P2X7 receptor. Following I/R, neuronal density decreased by 22.6 % in P2X7 receptor-immunoreactive neurons, and decreased by 46.7, 38, 39.8, 21.7, and 20 % in NOS-, calbindin-, calretinin-, ChAT-, and HuC/D-immunoreactive neurons, respectively, at 6, 24, and 72 h and 1 week following injury compared to the control and sham groups. We also observed a 14 % increase in the neuronal cell body profile area of the NOS-immunoreactive neurons at 6 and 24 h post-I/R and a 14 % increase in ChAT-immunoreactive neurons at 1 week following I/R. However, the average size of the calretinin-immunoreactive neurons was reduced by 12 % at 6 h post-I/R and increased by 8 % at 24 h post-I/R. This work demonstrates that I/R is associated with a significant loss of different subpopulations of neurons in the myenteric plexus accompanied by morphological changes, all of which may underlie conditions related to intestinal motility disorder. (AU)

FAPESP's process: 08/05314-5 - Study of the chemical coding and P2X2 and P2X7 purina receptors of the ileum enteric nervous system of rats submitted to ischemia/reperfusion intestinaL of the ileal artery
Grantee:Patricia Castelucci
Support type: Regular Research Grants