Cyclooxygenase-2 inhibition does not impair block bone grafts healing in rabbit model

Author(s)

Moreschi, Eduardo ^{[1, 2]} ; Biguetti, Claudia Cristina ^[3] ; Comparim, Eliston ^[1] ; Holgado, Leandro De Andrade ^[4] ; Ribeiro-Junior, Paulo Domingos ^[4] ; Nary-Filho, Hugo ^{[4, 5]} ; Matsumoto, Mariza Akemi ^[4]

Affiliation

^[1] Univ Sagrado Coracao, Oral & Maxillofacial Surg Doctorals Course Progra, Jardim Brasil, BR-17011160 Bauru, SP - Brazil

^[2] Ctr Univ Maringa CESUMAR, Maringa, Parana - Brazil

^[3] Univ Sao Paulo, FOB USP, Bauru Sch Dent, Oral Biol Masters Course Program, Dept Biol Sci, BR-17012901 Bauru, SP - Brazil

^[4] Univ Sagrado Coracao, Dept Hlth Sci, Jardim Brasil, BR-17011160 Bauru, SP - Brazil

^[5] PI Branemark Inst Bauru, Bauru - Brazil

• Document type: Journal article
• Source: Journal of Molecular Histology; v. 44, n. 6, p. 723-731, DEC 2013.
• Web of Science Citations: 5

Abstract

Success of alveolar reconstructions using onlay autogenous block bone grafts depends on their adequate integration to the recipient bed influenced by a number of local molecules. Considering the fundamental role of cyclooxygenase (COX-2) in bone repair, the aim of this study was to analyze the effect of its inhibition in the integration of endochondral (EC) iliac crest, and intramembranous (IM) calvaria bone grafts. Thirty-two rabbits were divided into 4 groups: Calvaria Control (CC) and Iliac Control-treated with oral 0.9 % saline solution, and Calvarial-NSAID (C-NSAID) and Iliac-NSAID (I-NSAID) groups-treated with oral 6 mg/Kg non-steroidal anti-inflammatory drug etoricoxib. After 7, 14, 30 and 60 days the animals were euthanized and the specimens removed for histological, histomorphometric and immunohistochemistry analysis. At day 60, a tight integration of IM blocks could be seen with the presence of remodeling bone, whereas integration of EC grafts was mainly observed at the edges of the grafts. A significant higher percentage of bone matrix in the interface region of the CC grafts in comparison to C-NSAID only at day 14, whereas no differences were detected comparing the EC grafts. No differences were observed in Runx-2 and vascular endothelial growth factor (VEGF) immunolabeling when comparing CC and C-NSAID groups, while a significant weaker Runx-2 and VEGF labeling was detected in I-NSAID group at day 60. Although some influence was detected in osteogenesis, it is concluded that drug induced inhibition of COX-2 does not impair onlay bone grafts' healing of both embryologic origins in rabbits. (AU)

• FAPESP's process: 09/14989-9 - Influence of cyclooxygenase 2 enzyme on autogenous bone grafts repair: histomorphometric analysis in rabbits
• Grantee: Claudia Cristina Biguetti
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 08/11485-7 - Immunohistochemical analysis of autogenous bone grafts repair under low-level lasertherapy: study in rabbits
• Grantee: Mariza Akemi Matsumoto
• Support Opportunities: Regular Research Grants