Synthesis and characterization of silver nanoparticles using water-soluble starch and its antibacterial activity on Staphylococcus aureus

Author(s)

Valencia, German Ayala ^[1] ; de Oliveira Vercik, Luci Cristina ^[1] ; Ferrari, Rosana ^{[2, 3]} ; Vercik, Andres ^[1]

Affiliation

^[1] Univ Sao Paulo, Basic Sci Dept ZAB FZEA, BR-13635000 Pirassununga, SP - Brazil

^[2] Inst Santa Cruz, Dept Biol Sci, Ilheus - Brazil

^[3] Univ Campinas UNICAMP, Inst Biol, Dept Histol & Embriol, Campinas, SP - Brazil

• Document type: Journal article
• Source: STARCH-STÄRKE; v. 65, n. 11-12, p. 931-937, NOV 2013.
• Web of Science Citations: 18

Abstract

The eco-friendly biosynthesis of silver nanoparticles (AgNPs), using starch as reducing agent, was addressed in this work. Starch solutions with three different concentrations (0.5, 1.0, and 1.5%w/v), heated at 90 degrees C for 12h and stabilized with 100 mu L of NaOH 0.1M were used during the synthesis process. AgNPs were characterized by UV-visible (UV-Vis) spectrophotometry, SEM equipped with energy dispersive spectrometer and TEM. The antibacterial activity of the AgNPs was evaluated on Staphylococcus aureus using the disc diffusion technique (Kirby-Bauer method) relative to enrofloxacin antibiotic. Solutions containing AgNPs developed a yellow color and presented UV-Vis spectra characteristic for AgNPs, with surface plasmon resonance peaks between 411 and 414nm. Spherical-shape AgNPs were observed by TEM with sizes between 10 and 30nm. These silver nanoparticles displayed an antimicrobial activity against S. aureus in colloidal state and films. (AU)

• FAPESP's process: 10/50424-3 - Development and characterization of nanostructured films for conductimetric hydrogen peroxide biosensors
• Grantee: Andrés Vercik
• Support Opportunities: Regular Research Grants