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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Deciphering the synergism of endogenous glycoside hydrolase families 1 and 9 from Coptotermes gestroi

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Author(s):
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Franco Cairo, Joao Paulo L. [1, 2] ; Oliveira, Leandro C. [1, 3] ; Uchima, Cristiane A. [1] ; Alvarez, Thabata M. [1] ; Citadini, Ana Paula da S. [1] ; Cota, Junio [1] ; Leonardo, Flavia Costa [2, 4] ; Costa-Leonardo, Ana M. [5] ; Carazzolle, Marcelo F. [2] ; Costa, Fernando F. [4] ; Pereira, Goncalo A. G. [2] ; Squina, Fabio M. [1]
Total Authors: 12
Affiliation:
[1] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Ciencia & Tecnol Bioetanol CTBE, BR-13083970 Campinas, SP - Brazil
[2] Univ Estadual Campinas, UNICAMP, Lab Genom & Expressao, Dept Genet Evolucao & Bioagentes, Campinas, SP - Brazil
[3] Univ Estadual Paulista, UNESP, Dept Fis, Inst Biociencias Letras & Ciencias Exatas IBILCE, Sao Jose Do Rio Preto, SP - Brazil
[4] Univ Estadual Campinas, Ctr Hematol & Hemoterapia, UNICAMP, Campinas, SP - Brazil
[5] Univ Estadual Paulista, UNESP, Dept Biol, Inst Biociencias, Rio Claro - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Insect Biochemistry and Molecular Biology; v. 43, n. 10, p. 970-981, OCT 2013.
Web of Science Citations: 16
Abstract

Termites can degrade up to 90% of the lignocellulose they ingest using a repertoire of endogenous and symbiotic degrading enzymes. Termites have been shown to secrete two main glycoside hydrolases, which are GH1 (EC 3.2.1.21) and GH9 (EC 3.2.1.4) members. However, the molecular mechanism for lignocellulose degradation by these enzymes remains poorly understood. The present study was conducted to understand the synergistic relationship between GH9 (CgEG1) and GH1 (CgBG1) from Coptotermes gestroi, which is considered the major urban pest of Sao Paulo State in Brazil. The goal of this work was to decipher the mode of operation of CgEG1 and CgBG1 through a comprehensive biochemical analysis and molecular docking studies. There was outstanding degree of synergy in degrading glucose polymers for the production of glucose as a result of the endo-beta-1,4-glucosidase and exo-beta-1,4-glucosidase degradation capability of CgEG1 in concert with the high catalytic performance of CgBG1, which rapidly converts the oligomers into glucose. Our data not only provide an increased comprehension regarding the synergistic mechanism of these two enzymes for cellulose saccharification but also give insight about the role of these two enzymes in termite biology, which can provide the foundation for the development of a number of important applied research topics, such as the control of termites as pests as well as the development of technologies for lignocellulose-to-bioproduct applications. (c) 2013 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 11/20977-3 - Investigation of plant biomass conversion in termite Coptotermes gestroi complementary to glycosyl hydrolases aiming at bioproducts formation derived from lignocellulosic biomass
Grantee:João Paulo Lourenço Franco Cairo
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 11/13242-7 - Studies of enzymatic mechanisms for the improvement of bio-fuel production
Grantee:Leandro Cristante de Oliveira
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 12/19040-0 - Characterization of gene expression of Neurospora crassa in response to deconstruction of sugar cane bagasse
Grantee:Cristiane Akemi Uchima
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 11/08609-9 - Structure based models in the study of enzymes related to bioethanol production
Grantee:Ronaldo Júnio de Oliveira
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 10/11469-1 - Library generation for biomass-conversion enzymes from soil metagenome
Grantee:Thabata Maria Alvarez
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 08/58037-9 - Library generation for biomass-conversion enzymes from soil metagenome
Grantee:Fábio Márcio Squina
Support type: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants