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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Purification and characterization of antimicrobial peptides from fish isolate Carnobacterium maltaromaticum C2: Carnobacteriocin X and carnolysins A1 and A2

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Author(s):
Tulini, Fabricio L. [1] ; Lohans, Christopher T. [2] ; Bordon, Karla C. F. [1] ; Zheng, Jing [2] ; Arantes, Eliane C. [1] ; Vederas, John C. [2] ; De Martinis, Elaine C. P. [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Sao Paulo - Brazil
[2] Univ Alberta, Dept Chem, Edmonton, AB T6G 2G2 - Canada
Total Affiliations: 2
Document type: Journal article
Source: International Journal of Food Microbiology; v. 173, p. 81-88, MAR 3 2014.
Web of Science Citations: 10
Abstract

Carnobacterium maltaromaticum C2, isolated from Brazilian smoked fish (Surubim, Pseudoplatystoma sp.), was found to exert antimicrobial activity against Listeria monocytogenes, an important foodborne pathogen. In this study, the bacteriocins produced by C. maltaromaticum 0 were purified via an extraction with XAD-16 resin, a C18 solid phase extraction, followed by reversed-phase fast protein liquid chromatography. The purified active fractions were characterized using tandem mass spectrometry, permitting the identification of multiple bacteriocins. Carnobacteriocins BM1, B1, and a variant of carnobacteriocin B2 were all found, providing much of the antilisterial activity. Additionally, we herein report the first isolation of the previously predicted antimicrobial peptide carnobacteriocin X. Moreover, C. maltaromaticum C2 produces a novel two-component lantibiotic, termed carnolysin, homologous to enterococcal cytolysin. This lantibiotic is antimicrobially inactive when tested against the non-bacteriocinogenic strain C. maltaromaticum A9b -, likely requiring an additional proteolytic cleavage to reach maturity. (C) 2013 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 09/10530-1 - Characterization of the bacteriocin from Carnobacterium maltaromaticum C2 isolated from Brazillian smoked fish (surubim [Pseudoplatystoma sp.])
Grantee:Fabricio Luiz Tulini
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 09/11134-2 - Characterization of the bacteriocin from Carnobacterium maltaromaticum C2 isolated from Brazillian smoked fish (Surubim [Pseudoplatystoma sp.])
Grantee:Elaine Cristina Pereira de Martinis
Support Opportunities: Regular Research Grants