| Full text | |
| Author(s): |
Crivelente Horta, Maria Augusta
[1]
;
Vicentini, Renato
[1]
;
Delabona, Priscila da Silva
[2]
;
Laborda, Prianda
[1]
;
Crucello, Aline
[1]
;
Freitas, Sindelia
[2]
;
Kuroshu, Reginaldo Massanobu
[3, 4]
;
Polikarpov, Igor
[3]
;
da Cruz Pradella, Jose Geraldo
[2]
;
Souza, Anete Pereira
[5, 1]
Total Authors: 10
|
| Affiliation: | [1] Univ Campinas UNICAMP, CBMEG, Campinas, SP - Brazil
[2] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Bioethanol Sci & Technol Lab CTBE, Campinas, SP - Brazil
[3] Univ Sao Paulo, Phys Inst Sao Carlos, Sao Carlos, SP - Brazil
[4] Fed Univ Sao Paulo UNIFESP, Inst Sci & Technol, Sao Jose Dos Campos, SP - Brazil
[5] Univ Campinas UNICAMP, Dept Plant Biol, Inst Biol, Campinas, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | PLoS One; v. 9, n. 2 FEB 18 2014. |
| Web of Science Citations: | 14 |
| Abstract | |
Profiling the transcriptome that underlies biomass degradation by the fungus Trichoderma harzianum allows the identification of gene sequences with potential application in enzymatic hydrolysis processing. In the present study, the transcriptome of T. harzianum IOC-3844 was analyzed using RNA-seq technology. The sequencing generated 14.7 Gbp for downstream analyses. De novo assembly resulted in 32,396 contigs, which were submitted for identification and classified according to their identities. This analysis allowed us to define a principal set of T. harzianum genes that are involved in the degradation of cellulose and hemicellulose and the accessory genes that are involved in the depolymerization of biomass. An additional analysis of expression levels identified a set of carbohydrate-active enzymes that are upregulated under different conditions. The present study provides valuable information for future studies on biomass degradation and contributes to a better understanding of the role of the genes that are involved in this process. (AU) | |