Oral hairy leukoplakia diagnosis by Epstein-Barr virus in situ hybridization in liquid-based cytology

Author(s)

Braz-Silva, P. H. ^{[1, 2]} ; Santos, R. T. M. ^[3] ; Schussel, J. L. ^[4] ; Gallottini, M. ^[2]

Affiliation

^[1] Univ Sao Paulo, Virol Lab, Inst Trop Med, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Oral Pathol, Sch Dent, Sao Paulo - Brazil

^[3] Adolfo Lutz Inst, Div Pathol, Sao Paulo - Brazil

^[4] Erasto Gaertner Hosp, Dept Oral & Maxillofacial Surg, Curitiba, Parana - Brazil

• Document type: Journal article
• Source: CYTOPATHOLOGY; v. 25, n. 1, p. 21-26, FEB 2014.
• Web of Science Citations: 5

Abstract

ObjectiveTo establish a definitive diagnosis of oral hairy leukoplakia (OHL) by insitu hybridization for Epstein-Barr virus (EBV) detection with liquid-based cytology (LBC), using the ThinPrep((R)) Pap Test, and to compare its efficacy with the traditional method of performing biopsy. MethodsThirty-three individuals divided into three groups were included in this study. Group 1 consisted of 15 human immunodeficiency virus (HIV)-positive patients with a clinical and histopathological diagnosis of OHL on the lateral border of the tongue. Group 2 consisted of 10 HIV-positive individuals with neither OHL nor other oral lesions. Group 3 consisted of 10 immunocompetent HIV-negative individuals with neither OHL nor other oral lesions. For each patient from the three groups, exfoliative LBC was performed on the lateral border of the tongue using ThinPrep. For the patients from group 1, a 6-mm-diameter punch biopsy was obtained from the same anatomic site as the brush collection to confirm the diagnosis of OHL by histopathology with insitu hybridization. Slides were prepared for morphological cellular analysis using Papanicolaou (Pap) staining, and for EBV detection using insitu hybridization. ResultsThirteen of the 15 patients from group 1 were confirmed on punch biopsy as OHL, providing the gold standard for the study. The sensitivity of LBC followed by a Pap-stained smear was 62% and the specificity was 90%. The sensitivity of LBC followed by insitu hybridization was 100% and the specificity was 100%. ConclusionsExfoliative LBC associated with EBV insitu hybridization is a simple, effective and non-invasive diagnostic tool for OHL. (AU)

• FAPESP's process: 07/07741-5 - Detection of Epstein-Barr virus (EBV) in oral mucosa of immunocompromised pacients by in situ hybridization in liquid based cytology.
• Grantee: Marina Helena Cury Gallottini
• Support Opportunities: Regular Research Grants