Ultra-fast determination of caffeine, dipyrone, and acetylsalicylic acid by capillary electrophoresis with capacitively coupled contactless conductivity detection and identification of degradation products

Author(s)

Marra, Mariana Cardoso ^[1] ; Cunha, Rafael Rodrigues ^[1] ; Rajh Vidal, Denis Tadeu ^[2] ; Abarza Munoz, Rodrigo Alejandro ^[1] ; do Lago, Claudimir Lucio ^[2] ; Richter, Eduardo Mathias ^[1]

Affiliation

^[1] Univ Fed Uberlandia, Inst Quim, BR-2121 Uberlandia, MG - Brazil

^[2] Univ Sao Paulo, Inst Quim, BR-748 Sao Paulo - Brazil

• Document type: Journal article
• Source: Journal of Chromatography A; v. 1327, p. 149-154, JAN 31 2014.
• Web of Science Citations: 27

Abstract

Capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-(CD)-D-4) was used for fast, simultaneous determination of dipyrone (DIP), caffeine (CAF), and acetylsalicylic acid (ASA). In the same run and in less than 1 min, the degradation products from DIP and ASA were also detected. In addition, the usage of the CE-(CD)-D-4 system allowed, for the first time, the detection of methylamine as a degradation product of DIP. Capillary electrophoresis with electrospray mass spectrometry experiments were carried out in order to confirm the formation of methylamine. The limits of detection by CE-(CD)-D-4 were 5, 5, and 6 mu mol L-1 for CAF, DIP, and ASA, respectively. The proposed method was applied to the analysis of these compounds in pharmaceutical formulations with similar results to those achieved by HPLC (p<0.05). (C) 2013 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 12/06642-1 - Formation and properties of adducts of organic hydroxy-compounds with carbonate in aqueous medium and biological conditions
• Grantee: Claudimir Lucio Do Lago
• Support Opportunities: Regular Research Grants