Advanced search
Start date
Betweenand
Conteúdos relacionados
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Development and characterization of microsatellite markers for Brazilian four-eyed frogs (genus Pleurodema) endemic to the Caatinga biome

Full text
Author(s):
Thome, M. T. C. [1] ; Alexandrino, J. [2] ; Lopes, S. [3] ; Haddad, C. F. B. [1] ; Sequeira, F. [3]
Total Authors: 5
Affiliation:
[1] Univ Estadual Paulista, Inst Biociencias, Dept Zool, Rio Claro, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Ciencias Biol, Diadema, SP - Brazil
[3] Univ Porto, Ctr Invest Biodiversidade & Recursos Genet, Vairao - Portugal
Total Affiliations: 3
Document type: Journal article
Source: Genetics and Molecular Research; v. 13, n. 1, p. 1604-1608, 2014.
Web of Science Citations: 1
Abstract

We used pyrosequencing to develop microsatellite markers for the Brazilian four-eyed frog Pleurodema diplolister and tested the microsatellite markers for cross-amplification in its sister Pleurodema alium, which are both endemic species of the Caatinga biome in northeastern Brazil. We used multiplex sets to amplify and genotype 30 individuals of P. diplolister from three different populations and 10 individuals of P. alium from a single population. We successfully amplified 24 loci for P. diplolister, 13 of which we were able to amplify in P. alium. All loci were polymorphic. Significant deviations from the Hardy-Weinberg equilibrium and the presence of null alleles were only consistently detected at one locus (Pleu9). These markers will enable the study of geographic genetic diversity and evolutionary processes in these two Caatinga endemics, and the inclusion of genetic data for conservation planning of the Caatinga biome. (AU)

FAPESP's process: 12/50255-2 - Filogeografia de anfibios comuns em regioes pouco estudadas do brasil:a caatinga e a regiao subtropical.
Grantee:João Miguel de Barros Alexandrino
Support Opportunities: Regular Research Grants
FAPESP's process: 12/06611-9 - The history of the Caatinga: mapping genetic patterns and evolutionary processes in an endemic species using rapid evolution markers.
Grantee:Maria Tereza Chiarioni Thomé
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 08/50928-1 - Especiacao de anfibios anuros em ambientes de altitude.
Grantee:Célio Fernando Baptista Haddad
Support Opportunities: Research Projects - Thematic Grants