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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparative analysis of two low-level laser doses on the expression of inflammatory mediators and on neutrophils and macrophages in acute joint inflammation

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dos Santos, Solange Almeida [1] ; Araruna Alves, Ana Carolina [2] ; Pinto Leal-Junior, Ernesto Cesar [2, 3] ; Albertini, Regiane [2, 3] ; Vieira, Rodolfo de Paula [2, 4] ; Ligeiro, Ana Paula [3] ; Silva Junior, Jose Antonio [4] ; Camillo de Carvalho, Paulo de Tarso [2, 3]
Total Authors: 8
[1] Univ Nove Julho UNINOVE, Dept Phys Therapy, Sao Paulo - Brazil
[2] Univ Nove Julho UNINOVE, Postgrad Program Rehabil Sci, BR-01504001 Sao Paulo - Brazil
[3] Univ Nove Julho UNINOVE, Postgrad Program Biophoton Appl Hlth Sci, Sao Paulo - Brazil
[4] Univ Nove Julho UNINOVE, Postgrad Program Med, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Lasers in Medical Science; v. 29, n. 3, p. 1051-1058, MAY 2014.
Web of Science Citations: 30

Synovial membrane inflammation plays an important role in osteoarthritis (OA) pathophysiology. The synovial tissue of patients with initial OA is characterized by mononuclear cell infiltration and the production of pro-inflammatory cytokines and other mediators of joint injury. The study aims to evaluate the effect of low-level laser therapy (LLLT) at doses of 2 and 4 J on joint inflammation in rats induced by papain through histopathological analysis, differential counts of inflammatory cells; gene expression of IL-1 beta, IL-6, and IL-10; and TNF-alpha protein expression. Male Wistar rats (20) were randomly divided (5 animals each) into a negative control group, an inflammation injury positive control group, a 2-J LLLT group subjected to injury and treated with 2 J of LLLT, and a 4-J LLLT group subjected to injury and treated with 4 J of LLLT. The animals were subjected to joint inflammation (4 % papain solution) and treated with LLLT. On the day of euthanasia, articular lavage was collected and centrifuged. The supernatant was analyzed for TNF-alpha protein expression by ELISA and IL-1 beta, IL-6, and IL-10 mRNA by RT-PCR. The joint tissue was also examined histologically. ANOVA with Tukey's post hoc test was used for comparisons. All data were expressed as means +/- S.D. (p < 0.05). Both laser modalities were efficient in reducing cellular inflammation and decreasing the expression of IL-1 beta and IL-6. However, the 2-J treatment led to more reduction in TNF-alpha than the 4-J treatment. A single application of LLLT with 2 J was more efficient in modulating inflammatory mediators and inflammatory cells. (AU)

FAPESP's process: 11/04834-8 - Effects of low level laser radiation with wavelengths of 660nm and 808nm on cartilage injury
Grantee:Ana Carolina Araruna Alves
Support type: Scholarships in Brazil - Master
FAPESP's process: 10/52404-0 - Low-level laser therapy in skeletal muscle fatigue and post-exercise recovery: Optimal parameters and effects in long-duration exercise
Grantee:Ernesto Cesar Pinto Leal Junior
Support type: Research Grants - Young Investigators Grants