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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Maturation of mononuclear phagocytes in the lungs of young calves-In vitro study

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Author(s):
Batista, C. F. [1] ; Blagitz, M. G. [2] ; Santos, B. P. [3] ; Bertagnon, H. G. [4] ; Parra, A. C. [5] ; Vianna, R. S. [6] ; de Lucca, G. G. [7] ; Lima, D. M. [8] ; Santos, D. S. [9] ; Della Libera, A. M. M. P. [10]
Total Authors: 10
Affiliation:
[1] Univ Sao Paulo. Fac Vet Med & Anim Sci FMVZ USP
[2] Univ Sao Paulo. Fac Vet Med & Anim Sci FMVZ USP
[3] Univ Sao Paulo. Fac Vet Med & Anim Sci FMVZ USP
[4] Univ Sao Paulo. Fac Vet Med & Anim Sci FMVZ USP
[5] Univ Sao Paulo. Fac Vet Med & Anim Sci FMVZ USP
[6] Univ Sao Paulo. Fac Vet Med & Anim Sci FMVZ USP
[7] Univ Sao Paulo. Fac Vet Med & Anim Sci FMVZ USP
[8] Univ Sao Paulo. Fac Vet Med & Anim Sci FMVZ USP
[9] Univ Sao Paulo. Fac Vet Med & Anim Sci FMVZ USP
[10] Univ Sao Paulo. Fac Vet Med & Anim Sci FMVZ USP
Total Affiliations: 10
Document type: Journal article
Source: JOURNAL OF DAIRY SCIENCE; v. 95, n. 10, p. 5909-5915, OCT 2012.
Web of Science Citations: 1
Abstract

The influences of age in calves' immune system are described in their first phase of life. We hypothesized that variations that occur in the main mechanisms of lung innate response can help to identify periods of greater susceptibility to the respiratory diseases that affect calves in the first stage of their life. This study aimed to evaluate the innate immune system. Nine healthy calves were monitored for 3 mo and 8 immunologic evaluations were performed. Bronchoalveolar lavage samples were recovered by bronchoscopy. The alveolar macrophages in samples were identified by protein expression of cluster of differentiation 14 (CD14) and underwent functional evaluation of phagocytosis (Staphylococcus aureus stained with propidium iodide and Escherichia coli). Data was assessed by one-way ANOVA (unstacked and parametric) and the Mann-Whitney test (nonparametric). Functional alterations in CD14-positive phagocytes were observed, with punctual higher intensity of phagocytosis in the third week and its decrease starting at 45 d of life. A gradual increase in phagocytosis rate was observed starting at this date. It is concluded that from 45 d of life on, alveolar macrophages have less phagocytic capacity but more cells perform this function. We suggest that this occurs because lung macrophages of calves start to maintain their immune response without passive immunity influence. Until 90 d of life, calves did not achieve the stability to conclude the maturation of local innate immune response. (AU)

FAPESP's process: 09/06013-1 - Study on maturation of innate immune response of the respiratory system in calves
Grantee:Alice Maria Melville Paiva Della Libera
Support Opportunities: Regular Research Grants