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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Beta-lactamase detection in Staphylococcus aureus and coagulase-negative Staphylococcus isolated from bovine mastitis

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Author(s):
Robles, Bruno F. [1] ; Nobrega, Diego B. [1] ; Guimaraes, Felipe F. [1] ; Wanderley, Guido G. [1] ; Langoni, H. [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Paulista Unesp, Dept Higiene Vet & Saude Publ, BR-18618900 Botucatu, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Pesquisa Veterinária Brasileira; v. 34, n. 4, p. 325-328, APR 2014.
Web of Science Citations: 2
Abstract

The objectives of the study were to evaluate the presence/production of beta-lactamases by both phenotypic and genotypic methods, verify whether results are dependent of bacteria type (Staphylococcus aureus versus coagulase-negative Staphylococcus - CNS) and verify the agreement between tests. A total of 200 bacteria samples from 21 different herds were enrolled, being 100 CNS and 100 S. aureus. Beta-lactamase presence/detection was performed by different tests (PCR, clover leaf test - CLT, Nitrocefin disk, and in vitro resistance to penicillin). Results of all tests were not dependent of bacteria type (CNS or S. aureus). Several S. aureus beta-lactamase producing isolates were from the same herd. Phenotypic tests excluding in vitro resistance to penicillin showed a strong association measured by the kappa coefficient for both bacteria species. Nitrocefin and CLT are more reliable tests for detecting beta-lactamase production in staphylococci. (AU)

FAPESP's process: 11/08533-2 - Beta-lactamases detection in Staphylococcus aureus and coagulase negative Staphylococcus strains isolated from bovine mastitis
Grantee:Bruno Ferreira Robles
Support Opportunities: Scholarships in Brazil - Scientific Initiation