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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In vitro schistosomicidal effects of aqueous and dichloromethane fractions from leaves and stems of Piper species and the isolation of an active amide from P. amalago L. (Piperaceae)

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Author(s):
Carrara, V. S. [1] ; Vieira, S. C. H. [1] ; de Paula, R. G. [2] ; Rodrigues, V. [2] ; Magalhaes, L. G. [3] ; Cortez, D. A. G. [1] ; Da Silva Filho, A. A. [4]
Total Authors: 7
Affiliation:
[1] Univ Estadual Maringa, Dept Farm, Maringa, Parana - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, BR-14049 Ribeirao Preto, SP - Brazil
[3] Univ Franca, Nucleo Pesquisas Ciencias Exatas & Tecnol, Franca, SP - Brazil
[4] Univ Fed Juiz de Fora, Dept Ciencias Farmaceut, Fac Farm, Juiz De Fora, MG - Brazil
Total Affiliations: 4
Document type: Journal article
Source: JOURNAL OF HELMINTHOLOGY; v. 88, n. 3, p. 321-326, SEP 2014.
Web of Science Citations: 12
Abstract

Dichloromethane and aqueous fractions from leaves and stems of Piper arboreum Aubl., P. aduncum L., P. amalago L., P. crassinervium H. B. \& K., P. diospyrifolium Kunth, P. hispidum Sw. and P. xylosteoides (Kunth) Steud. were tested against adult worms of Schistosoma mansoni. The in vitro activity was evaluated in terms of mortality, number of separated worms and number of worms with reduced motor activity. Most dichloromethane fractions from all Piper species showed moderate schistosomicidal activity, but aqueous fractions were not active. The dichloromethane fraction of P. amalago leaves (at 100 mu g/ml) showed the highest activity, resulting in worm mortality, the separation of worm pairs and reduced motor activity. Chromatographic fractionation of the dichloromethane fraction of P. amalago leaves led to the isolation of its major compound, which was also tested against adults of S. mansoni. The isolated piperamide N-{[}7-(3',4'-methylenedioxyphenyl)-2(Z), 4(Z)-heptadienoyl] pyrrolidine, at 100 mu M, resulted in the mortality of all adult worms after 24 h of incubation. The findings suggest that species of Piper are potential sources of schistosomicidal compounds. (AU)