Immunochemical methods in the study of microorganisms capable to hydrolise alergenic proteins in plant foods

• Grant number: 18/11475-3
• Support Opportunities: Research Grants - Visiting Researcher Grant - International
• Start date: September 17, 2018
• End date: December 16, 2018
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Principal Investigator: Bernadette Dora Gombossy de Melo Franco
• Grantee: Bernadette Dora Gombossy de Melo Franco
• Visiting researcher: Kamel Eddine El Mecherfi
• Visiting researcher institution: Institut National de la Recherche Agronomique, Nantes (INRA), France
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 13/07914-8 - FoRC - Food Research Center, AP.CEPID

Abstract

The present proposal aims to receive the researcher Dr. Kamel Eddine El Mecherfi from the Biopolymères Interactions Assemblages of the Institut National de la Recherche Agronomique (INRA) in Nantes, France, as a visiting researcher for a period of three months, to carry out research and teaching activities related to the project "Reduction of allergenicity of food proteins by the use of proteolytic microorganisms", underway at CEPID Food Research Center (FoRC). The proposal aims to continue an on-going partnership between the FoRC research group in the area of food microbiology and researchers at the BIA/INRA laboratory, which is currently focused on the study of food-borne lactic bacteria capable of reducing allergenicity of protein from soy and wheat during the fermentation process. This is a new approach in the topic of food allergies, which is a major challenge for the food industry, which increasingly needs ingredients of plant origin. During the visit, Dr. El Mecherfi will perform laboratory tests with the proteolytic bacterial strains available, using different substrates based on soy and wheat and will standardize the immunochemical methodologies such as dot-blot and direct and indirect ELISA, used in the detection of residual allergens in the products fermented by these bacteria. In addition to working in the laboratory assisting a graduate and postdoctoral fellow involved in this project, Dr. El Mecherfi will teach a specific course on the scientific basis of these and other techniques used in the study of immunogenic proteins, as well as participate in other disciplines in the postgraduate programs in Food Science and Biochemical and Pharmaceutical Technology, both from FCF/USP. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

EL MECHERFI, KAMEL-EDDINE; LUPI, ROBERTA; CHERKAOUI, MEHDI; ALBUQUERQUE, MARCELA A. C.; TODOROV, SVETOSLAV DIMITROV; TRANQUET, OLIVIER; KLINGEBIEL, CAROLINE; ROGNIAUX, HELENE; DENERY-PAPINI, SANDRA; ONNO, BERNARD; et al. Fermentation of Gluten by Lactococcus lactis LLGKC18 Reduces its Antigenicity and Allergenicity. PROBIOTICS AND ANTIMICROBIAL PROTEINS, JUN 2021. (18/11475-3)

EL MECHERFI, KAMEL-EDDINE; TODOROV, SVETOSLAV DIMITROV; CAVALCANTI DE ALBUQUERQUE, MARCELA ALBUQUERQUE; DENERY-PAPINI, SANDRA; LUPI, ROBERTA; HAERTLE, THOMAS; DORA GOMBOSSY DE MELO FRANCO, BERNADETTE; LARRE, COLETTE. Allergenicity of Fermented Foods: Emphasis on Seeds Protein-Based Products. FOODS, v. 9, n. 6, JUN 2020. (13/07914-8, 18/10540-6, 18/11475-3)

EL MECHERFI, KAMEL-EDDINE; LUPI, ROBERTA; CHERKAOUI, MEHDI; ALBUQUERQUE, MARCELA A. C.; TODOROV, SVETOSLAV DIMITROV; TRANQUET, OLIVIER; KLINGEBIEL, CAROLINE; ROGNIAUX, HELENE; DENERY-PAPINI, SANDRA; ONNO, BERNARD; et al. Fermentation of Gluten by Lactococcus lactis LLGKC18 Reduces its Antigenicity and Allergenicity. PROBIOTICS AND ANTIMICROBIAL PROTEINS, v. 14, n. 5, p. 13-pg., 2021-06-03. (18/11475-3)