Effects of aerobic physical training and the availability of wide space housing on the spontaneous physical activity, central and peripheral metabolic and molecular responses

• Grant number: 17/10201-4
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2018
• End date: April 30, 2020
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Claudio Alexandre Gobatto
• Grantee: Claudio Alexandre Gobatto
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated researchers: Fúlvia de Barros Manchado Gobatto ; Ivan Gustavo Masselli dos Reis ; Tomas Alberto Prolla

Abstract

Recent attention has been given to the role of spontaneous physical activity (SPA), which represents all non-voluntary physical activities of daily life, such as posture maintenance, ambulation, and muscle restlessness. Considering the strong influence of SPA on metabolism, it is possible that animals with higher SPA have an efficient energetic and acid-base control in hypothalamic and skeletal muscle tissues, which seem to be involved in the SPA control and motor performance. However, studies involving these aspects without use of genetic or pharmacological manipulations are still non-existent in the scientific literature. We have evidence that the association of aerobic physical training with the maintenance of animals in cages of wide housing space can provide an interesting experimental model on knowledge of how AFE manifests itself in rodents and, thus, to establish a better developmental understanding, which obviously involves the performance and longevity. Thus, the objective of this study is to investigate the effects of aerobic physical training and the availability of wide space housing on the SPA and central (hypothalamus) and peripheral (skeletal musculature) metabolic responses. Aiming to evaluate the central SPA control, the hypothalamic protein content of orexin receptors (OX1R and OX2R) will be determined. In this tissue will be also quantified the protein expressions (content) of neuropeptide Y (NPY) and Agouti-related peptide (AgRP), which seem to play role in the food consumption regulation and energy balance, and monocarboxylic transporters (MCTs), isoforms 1 , 2 and 4, which probably in the hypothalamus exert control of acid-base balance. To the peripheral analysis, the protein expressions (content) of OX1R, OX2R, MCT-1 and MCT-4 in the soleus (oxidative) and gastrocnemius muscles (white, glycolytic portion) will be quantified. Finally, the lipid, glycidic and biometric profile of the animals will be determined. Thus, forty C57BL / 6J mice will be divided into two types of housing: standard cage (HP, 166 cm2 per animal) or wide space housing (HA, 480 cm2 per animal). For each type of housing, the mice will be subdivided into two groups: control (C) or trained (T). The T-group mice will undergone a running aerobic training program, which must be performed at 80% of the critical velocity. The total duration of each exercise session (daily volume) will be 40 minutes with weekly frequency of five days. Throughout the experimental period, there will be determined the behavioral of the animals by the SPA (in loco) from a gravimetric system (in both housings). Before, after the 4th week and at the end of the exercise (8th week), the aerobic and anaerobic capacities will be evaluated by the velocity critical protocol. At the end of week 8, the animals will be euthanized for withdrawal the samples of the hypothalamus, and soleus and gastrocnemius muscles. We hypothesize that animals housed in a wide housing space, especially trained animals, will present: high SPA, higher protein content of OX1R and OX2R receptors, as well as MCTs 1, 2 and 4 and hypothalamic NPY and AgRP; Increased food intake and higher protein content of OX1R, OX2R, MCT-1 and MCT-4 in skeletal muscles; lower and larger masses of white and brown adipose tissue, respectively. We hypothesize also greater lean mass and superior aerobic and anaerobic capacities, with optimized stocks of hepatic and muscular glycogen, as well as circulating energy substrates. Considering the relevance of SPA, it is undeniable the importance to establish an understanding about the relationships among SPA and central and peripheral metabolic and molecular responses. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SCARIOT, PEDRO P. M.; MANCHADO-GOBATTO, FULVIA B.; VAN GINKEL, PAUL R.; PROLLA, TOMAS A.; GOBATTO, CLAUDIO A.. Aerobic training associated with an active lifestyle exerts a protective effect against oxidative damage in hypothalamus and liver: The involvement of energy metabolism. Brain Research Bulletin, v. 175, p. 116-129, OCT 2021. (17/10201-4, 19/05115-7, 15/01362-9, 15/00272-6)

SCARIOT, PEDRO PAULO MENEZES; PAPOTI, MARCELO; POLISEL, EMANUEL ELIAS CAMOLESE; ORSI, JUAN BORDON; GINKEL, PAUL R. VAN; PROLLA, TOMAS A.; MANCHADO-GOBATTO, FULVIA BARROS; GOBATTO, CLAUDIO ALEXANDRE. Living high- training low model applied to C57BL/6J mice: Effects on physiological parameters related to aerobic fitness and acid-base balance. Life Sciences, v. 317, p. 17-pg., 2023-02-01. (17/10201-4, 15/00272-6, 15/01362-9, 19/05115-7, 16/12781-5)

SCARIOT, P. P. M.; MANCHADO-GOBATTO, F. B.; BECK, W. R.; PAPOTI, M.; VAN GINKEL, P. R.; GOBATTO, C. A.. Monocarboxylate transporters (MCTs) in skeletal muscle and hypothalamus of less or more physically active mice exposed to aerobic training. Life Sciences, v. 307, p. 11-pg., 2022-08-11. (18/07099-6, 17/10201-4, 19/05115-7, 15/01362-9, 15/00272-6, 14/10336-9)

MENEZES SCARIOT, PEDRO PAULO; MANCHADO-GOBATTO, FULVIA B.; PROLLA, TOMAS A.; MASSELLI DOS REIS, IVAN G.; GOBATTO, CLAUDIO ALEXANDRE. Housing conditions modulate spontaneous physical activity, feeding behavior, aerobic running capacity and adiposity in C57BL/6J mice. Hormones and Behavior, v. 115, SEP 2019. (17/10201-4, 15/00272-6, 15/01362-9)

SCARIOT, PEDRO P. M.; GOBATTO, CLAUDIO A.; POLISEL, EMANUEL E. C.; GOMES, ANA E. C.; BECK, WLADIMIR R.; MANCHADO-GOBATTO, FULVIA B.. Early-life mice housed in standard stocking density reduce the spontaneous physical activity and increase visceral fat deposition before reaching adulthood. LABORATORY ANIMALS, v. N/A, p. 12-pg., 2022-01-21. (17/10201-4, 14/10336-9, 19/08148-3, 15/01362-9, 15/00272-6, 18/07099-6, 19/05115-7)

POLISEL, EMANUEL E. C.; BECK, WLADIMIR R.; SCARIOT, PEDRO P. M.; PEJON, TACIANE M. M.; GOBATTO, CLAUDIO A.; MANCHADO-GOBATTO, FULVIA B.. Effects of high-intensity interval training in more or less active mice on biomechanical, biophysical and biochemical bone parameters. SCIENTIFIC REPORTS, v. 11, n. 1, MAR 19 2021. (15/00272-6, 18/07099-6, 19/05115-7, 15/01362-9, 19/08148-3, 14/10336-9, 17/10201-4)