Multi user equipment approved in grant 2017/50.339-5: nanoespectrofotômetro BioDrop

• Grant number: 18/19452-2
• Support Opportunities: Multi-user Equipment Program
• Start date: November 01, 2018
• End date: October 31, 2025
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Production
• Principal Investigator: Renata Helena Branco Arnandes
• Grantee: Renata Helena Branco Arnandes
• Host Institution: Instituto de Zootecnia. Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Nova Odessa , SP, Brazil
• Associated research grant: 17/50339-5 - Institutional research development plan of the Animal Science Institute (PDIp), AP.PDIP
• EMU web page: http://www.iz.sp.gov.br/pagina.php?id=100
• Type of equipment: Caracterização de Materiais - Imageamento - Ótico; Caracterização de Materiais - Espectroscopia - Óptica (UV-Visível)
• Manufacturer: Biodrop
• Model: BioDrop ullTE UV/VIS

Abstract

The BioDrop Spectrophotometer is a DNA, RNA and protein quantifier necessary for the initial stage of several analyzes because it is necessary to extract the DNA and its quantification to continue the other analyzes of interest, such as: genotyping and selection of characters of economic interest, genetic identification of animals, genetic identification of pathogenic microorganisms. Currently DNA quantification is performed by application of agarose gel sample and comparison with molecular weight standard. This type of quantification (with agarose gel) does not give exact results besides being more laborious, it spends about 40 minutes and it spends much more DNA previously extracted. With the acquisition of this equipment, the quantification will be quicker (immediate result), accurate, economical through the use of only 1 microliter of sample containing DNA, whereas in the currently used method (with agarose gel), 10 microliter of sample. It is expected that with the acquisition of the Biodrop equipment the genetic analyzes will be optimized in relation to the time of preparation and execution of the test, as well as the reduction of waste of materials and work used for the purification of the genetic material through the optimization of the volume of use of the material (DNA or RNA) to be analyzed. With this, a larger number of samples can be analyzed and more volume of search results will be achieved. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AZEVEDO, BIANCA TAINA; VERCESI FILHO, ANIBAL EUGENIO; GUTMANIS, GUNTA; VERISSIMO, CECILIA JOSE; KATIKI, LUCIANA MORITA; OKINO, CINTIA HIROMI; DE SENA OLIVEIRA, MARCIA CRISTINA; GIGLIOTI, RODRIGO. New sensitive methods for fraud detection in buffalo dairy products. INTERNATIONAL DAIRY JOURNAL, v. 117, JUN 2021. (18/19452-2)

PACHECO, POLIANA ARAUJO; LOUVANDINI, HELDER; GIGLIOTI, RODRIGO; RODRIGUES WEDY, BRUNA COSTA; RIBEIRO, JESSICA CAROLINA; VERISSIMO, CECILIA JOSE; FERREIRA, JORGE FREIRE DA SILVA; TALAMINI DO AMARANTE, ALESSANDRO FRANCISCO; KATIKI, LUCIANA MORITA. Phytochemical modulation of P-Glycoprotein and its gene expression in an ivermectin-resistant Haemonchus contortus isolate in vitro. Veterinary Parasitology, v. 305, p. 9-pg., 2022-05-01. (17/23540-1, 18/02423-0, 18/19452-2, 18/15206-7)

GIGLIOTI, RODRIGO; OKINO, CINTIA HIROMI; AZEVEDO, BIANCA TAINA; GUTMANIS, GUNTA; KATIKI, LUCIANA MORITA; DE SENA OLIVEIRA, MARCIA CRISTINA; VERCESI FILHO, ANIBAL EUGENIO. Novel LNA probe-based assay for the A1 and A2 identification of beta-casein gene in milk samples. FOOD CHEMISTRY: MOLECULAR SCIENCES, v. 3, p. 6-pg., 2021-12-30. (18/19452-2)

GIGLIOTI, RODRIGO; DA SILVA FERREIRA, JORGE FREIRE; LUCIANI, GUILHERME FAVERO; LOUVANDINI, HELDER; OKINO, CINTIA HIROMI; NICIURA, SIMONE CRISTINA MEO; DE SENA OLIVEIRA, MARCIA CRISTINA; DO AMARANTE, ALESSANDRO FRANCISCO TALAMINI; KATIKI, LUCIANA MORITA. Potential of Haemonchus contortus first-stage larvae to characterize anthelmintic resistance through P-glycoprotein gene expression. Small Ruminant Research, v. 217, p. 6-pg., 2022-11-10. (19/26042-8, 18/19452-2)

FRABETTI, ACUCENA FRAGNAN; KATIKI, LUCIANA MORITA; CAETANO, LAURA; SARTI, MAYNE BARBOZA; FALASCA, THAMIRES MAROCCI; POLLI, HIAGO; VERISSIMO, CECILIA JOSE; VERCESI FILHO, ANIBAL EUGENIO; DE OLIVEIRA, HENRIQUE NUNES; OLIVEIRA, MARCIA CRISTINA DE SENA; et al. Natural levels of Rhipicephalus microplus infestation and Anaplasma marginale infection in Angus and Ultrablack calves. Experimental and Applied Acarology, v. 89, n. 1, p. 10-pg., 2023-01-05. (19/22675-6, 18/19452-2)

GIGLIOTI, RODRIGO; OKINO, CINTIA HIROMI; AZEVEDO, BIANCA TAINA; RODRIGUES WEDY, BRUNA COSTA; GUTMANIS, GUNTA; VERISSIMO, CECILIA JOSE; KATIKI, LUCIANA MORITA; VERCESI FILHO, ANIBAL EUGENIO; DE OLIVEIRA, HENRIQUE NUNES; DE SENA OLIVEIRA, MARCIA CRISTINA. Semi-quantitative evaluation of Babesia bovis and B. bigemina infection levels estimated by HRM analysis. TICKS AND TICK-BORNE DISEASES, v. 12, n. 5, SEP 2021. (19/22675-6, 16/07216-7, 18/19452-2)