CONCURRENT TRAINING: EFFECT ON EPIGENETIC MECHANISM OF DNA METHILATION

• Grant number: 18/12150-0
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2019
• End date: August 31, 2021
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Cleiton Augusto Libardi
• Grantee: Cleiton Augusto Libardi
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated researchers: Carlos Ugrinowitsch

Abstract

Introduction: Acute responses of DNA methylation levels in myogenic and satellite cell activation (CSs) genes may be the mechanisms that explain the lower gain of muscle mass after a period of endurance training (ET) associated with resistance training (RT) (i.e., concurrent training [CT]) compared to RT only. Purpose: To determine and compare the changes induced by a session RT, ET and CT in DNA methylation levels and expression of genes related to myogenic satellite cells and mitochondrial biogenesis. Methods: Fifteen untrained men will perform three different training sessions, namely: RT, ET and CT, in a randomized and counterbalanced way (cross-over experimental design), with interval of one week between them. RT: leg press 45° and leg extension exercise with 2 sets of 8-12 maximal repetitions with 60s rest; ET: 12 sprints of 1 min at 100% of vVO2max with interval of 1 minute at 50% of vVO2max between sprints; CT: TF protocol followed by the TA protocol, with a 5-minute interval between them. Four samples will be obtained from blood and muscle tissue for each experimental session, one collected immediately before exercise (Pre) and another three collected after exercise (immediately, after 4h and 8h). Methylation levels of genes related to myogenesis (MyoD, Myf5, MyoG, MRF4) and mitochondrial biogenesis (PGC-1±, TFAM, PPAR-³) will be determined and compared, as well as the amount and state (quiescence, or differentiation) of the satellite cells. Hypothesis: We hope that the RT session will produce the demethylation of the myogenic genes, and the consequent increase in mRNA transcription of these genes, the ET session, on the contrary, will increase the methylation of the myogenic genes by decreasing the transcription of the respective mRNAs. Thus, we also hypothesized that CT will attenuate the demethylation of myogenic genes in comparison to the isolated RT, minimizing the expression of these genes and, consequently, in the cycle of activation, proliferation and differentiation of CSs, a factor that can induce the effect of interference observed after a period of CT. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VECHIN, FELIPE C.; CONCEICAO, MIGUEL S.; TELLES, GUILHERME D.; LIBARDI, CLEITON A.; UGRINOWITSCH, CARLOS. Interference Phenomenon with Concurrent Strength and High-Intensity Interval Training-Based Aerobic Training: An Updated Model. SPORTS MEDICINE, v. 51, n. 4, JAN 2021. (18/12150-0)

TELLES, GUILHERME DEFANTE; LIBARDI, CLEITON AUGUSTO; CONCEICAO, MIGUEL SOARES; VECHIN, FELIPE CASSARO; LIXANDRAO, MANOEL EMILIO; ROTEA MANGONE, FLAVIA REGINA; PAVANELLI, ANA CAROLINA; NAGAI, MARIA APARECIDA; CAMERA, DONNY MICHAEL; HAWLEY, JOHN A.; et al. Interrelated but Not Time-Aligned Response in Myogenic Regulatory Factors Demethylation and mRNA Expression after Divergent Exercise Bouts. MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, v. 55, n. 2, p. 10-pg., 2023-02-01. (18/12150-0, 18/16513-0, 17/01297-8)

TELLES, GUILHERME DEFANTE; LIBARDI, CLEITON AUGUSTO; CONCEICAO, MIGUEL SOARES; VECHIN, FELIPE CASSARO; LIXANDRAO, MANOEL EMILIO; LUGNANI DE ANDRADE, ANDRE LUIS; GUEDES, DANIEL NOVAIS; UGRINOWITSCH, CARLOS; CAMERA, DONNY MICHAEL. Time Course of Skeletal Muscle miRNA Expression after Resistance, High-Intensity Interval, and Concurrent Exercise. MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, v. 53, n. 8, p. 1708-1718, AUG 2021. (18/12150-0, 18/16513-0, 17/01297-8)