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Development of glucose biosensor as an analytical tool aiming the optimization of second-generation ethanol production from pineapple residues

Abstract

Bioethanol is a fuel derived from renewable energy sources. However, the main production processes use biomasses that are also used also as food. For this reason, new technologies are needed that use alternative sources as a raw material, such as the production of second-generation (2G) ethanol, which uses lignocellulosic biomass in its process. This biomass can be in the form of bagasse, straw, bark, in other words, products that are residues from the agricultural chain production. In addition, these residues can be plants that in some way cause problems (eg. weeds), like the water hyacinth that in a short time can cover water bodies, causing its eutrophication. An example of agricultural waste that may contribute toward is the pineapple residues. Is a vegetal biomass and contains in its structure cellulose that can be degraded into glucose and later used as substrate for the production of ethanol. However, during the enzymatic hydrolysis of cellulose, glucose becomes a limiting factor, since its accumulation in the bioreactor causes inhibition in the cellulase enzyme, and this implies in a lower production of this hexose consequently a lower yield in ethanol production. Therefore, it is proposed the development of sensors associated with the electrochemical potential of graphene and the specificity of glucose enzyme for the monitoring and quantification of glucose in the 2G ethanol production process. (AU)

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Scientific publications (8)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SETZNAGL, SARAH; CESARINO, IVANA. Copper nanoparticles and reduced graphene oxide modified a glassy carbon electrode for the determination of glyphosate in water samples. INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, v. 102, n. 1, p. 293-305, JAN 2 2022. Web of Science Citations: 5.
SILVA, MARTIN K. L.; SOUSA, GUILHERME S.; SIMOES, RAFAEL P.; CESARINO, IVANA. Fabrication of paper-based analytical devices using a PLA 3D-printed stencil for electrochemical determination of chloroquine and escitalopram. Journal of Solid State Electrochemistry, NOV 2021. Web of Science Citations: 0.
ZACCARIOTTO, GABRIEL C.; SILVA, MARTIN K. L.; ROCHA, GIOVANNA S.; CESARINO, IVANA. A Novel Method for the Detection of SARS-CoV-2 Based on Graphene-Impedimetric Immunosensor. MATERIALS, v. 14, n. 15 AUG 2021. Web of Science Citations: 0.
SILVA, MARTIN K. L.; CESARINO, IVANA. Electrochemical sensor based on Sb nanoparticles/reduced graphene oxide for heavy metal determination. INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, MAY 2020. Web of Science Citations: 0.
SETZNAGL, SARAH; CESARINO, IVANA. Copper nanoparticles and reduced graphene oxide modified a glassy carbon electrode for the determination of glyphosate in water samples. INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, FEB 2020. Web of Science Citations: 0.
FERNANDES, VICTORIA QUINTAS; LEME SILVA, MARTIN K.; CESARINO, IVANA. Determination of isotretinoin (13-cis-retinoic acid) using a sensor based on reduced graphene oxide modified with copper nanoparticles. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, v. 856, JAN 1 2020. Web of Science Citations: 0.
DONINI, CARUANE A.; SILVA, MARTIN K. L.; BRONZATO, GIOVANA R.; LEAO, ALCIDES L.; CESARINO, IVANA. Evaluation of a biosensor based on reduced graphene oxide and glucose oxidase enzyme on the monitoring of second-generation ethanol production. Journal of Solid State Electrochemistry, v. 24, n. 8, SI DEC 2019. Web of Science Citations: 1.
BRONZATO, GIOVANA R. F.; DOS REIS, VICTOR A. C. A.; BORRO, JESSYCA A.; LEAO, ALCIDES L.; CESARINO, IVANA. Second generation ethanol made from coir husk under the biomass Cascade approach. MOLECULAR CRYSTALS AND LIQUID CRYSTALS, v. 693, n. 1, SI, p. 107-114, NOV 2 2019. Web of Science Citations: 0.

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