Influence of gamma-orizanol intervention on the prevention of cardiorreanal metabolic syndrome in obese rats: participation of protein carbonylation

• Grant number: 18/15294-3
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2019
• End date: September 30, 2021
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Camila Renata Corrêa
• Grantee: Camila Renata Corrêa
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Fabiane Valentini Francisqueti Ferron

Abstract

Cardiorenal metabolic syndrome (CRMS) is a pathological condition associated with the development of cardiac and renal dysfunction due to obesity and associated complications, in which oxidative stress plays an important role in triggering the carbonylation of proteins The gamma-oryzanol (³Oz), the main phytosterol present in rice grains, has demonstrated potential antiadipogenic, hypocholesterolemic, anti-inflammatory and antioxidant action. Therefore, the objective of this study is to evaluate the preventive effect of gamma-oryzanol on the carbonylation of proteins CRSM-associated. Male Wistar rats (n = 48), with 21 days of age, will be used, coming from the biothermium of Universidade Estadual Paulista (UNESP). The animals will be distributed in four experimental groups (n = 12 animals / group). The G1 group will receive standard diet (SD), G2 diet standard + gamma-orizanol (SD + yOz), G3 diet rich in carbohydrates and fat (CFD) and G4 diet rich in carbohydrates and fat + gamma-oryzanol (CFD + yOz) for 30 weeks. Consumption of chow and water will be monitored daily and body weight weekly. At the end of the experiment and prior to euthanasia, the evaluations of systolic blood pressure, echocardiogram and 24-hour urine collection to assess proteinuria and glomerular filtration rate. Fat deposits will be collected to characterize obesity in plasma to measure biochemical, hormonal and inflammatory parameters and the kidneys and heart to determine antioxidant activity, carbonyl species and proteomics (carbonylated proteins). In addition, the concentration of yOz in renal cardiac tissues and plasma by high performance cormatography (HPLC) will be measured. Parametric data will be presented as mean ± standard deviation and comparisons between groups will be performed by analysis of parametric variance (ANOVA) and complemented by the Tukey post-hoc test. Non-parametric data will be presented as median and interquartile (p25 and p75) and comparisons between groups will be performed by Kruskal-Wallis and complemented by Dunn's post-hoc test. Differences will be considered significant when p <0.05. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FRANCISQUETI-FERRON, FABIANE VALENTINI; TOGNERI FERRON, ARTUR JUNIO; GARCIA, JESSICA LEITE; VAGULA DE ALMEIDA SILVA, CAROL CRISTINA; COSTA, MARIANE ROVERO; GREGOLIN, CRISTINA SCHMITT; MORETO, FERNANDO; FERREIRA, ANA LUCIA A.; MINATEL, IGOR OTAVIO; CORREA, CAMILA RENATA. Basic Concepts on the Role of Nuclear Factor Erythroid-Derived 2-Like 2 (Nrf2) in Age-Related Diseases. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 20, n. 13, JUL 1 2019. (18/15294-3)

FRANCISQUETI-FERRON, FABIANE VALENTINI; GARCIA, JESSICA LEITE; TOGNERI FERRON, ARTUR JUNIO; NAKANDAKARE-MAIA, ERIKA TIEMI; GREGOLIN, CRISTINA SCHMITT; DAS CHAGAS SILVA, JANAINA PAIXAO; DOS SANTOS, KLINSMANN CAROLO; COLOMBO LO, ANGELO THOMPSON; SIQUEIRA, JULIANA SILVA; DE MATTEI, LETICIA; et al. Gamma-oryzanol as a potential modulator of oxidative stress and inflammation via PPAR-y in adipose tissue: a hypothetical therapeutic for cytokine storm in COVID-19?. Molecular and Cellular Endocrinology, v. 520, JAN 15 2021. (18/15294-3, 15/10626-0)

SIQUEIRA, JULIANA SILVA; VIEIRA, TAYNARA APARECIDA; NAKANDAKARE-MAIA, ERIKA TIEMI; NOVAGA PALACIO, THIAGO LUIZ; SARZI, FELIPE; GARCIA, JESSICA LEITE; DE PAULA, BRUNO HENRIQUE; ZANATI BAZAN, SILMEIA GARCIA; BARON, GIOVANNA; TUCCI, LUIGI; et al. Bergamot leaf extract treats cardiorenal metabolic syndrome and associated pathophysiological factors in rats fed with a high sugar fat diet. Molecular and Cellular Endocrinology, v. 556, p. 10-pg., 2022-08-05. (18/15294-3)

NAKANDAKARE-MAIA, ERIKA TIEMI; SIQUEIRA, JULIANA SILVA; FERRON, ARTUR JUNIO TOGNERI; VIEIRA, TAYNARA APARECIDA; PALACIO, THIAGO LUIZ NOVAGA; GRANDINI, NUBIA ALVES; GARCIA, JESSICA LEITE; BELIN, MATHEUS ANTONIO; ALTOMARE, ALESSANDRA; BARON, GIOVANNA; et al. Treatment with bergamot (Citrus bergamia) leaves extract attenuates leptin resistance in obese rats. Molecular and Cellular Endocrinology, v. 566, p. 7-pg., 2023-03-13. (18/15294-3)

FRANCISQUETI-FERRON, FABIANE VALENTINI; TOGNERI FERRON, ARTUR JUNIO; GARCIA, JESSICA LEITE; VAGULA DE ALMEIDA SILVA, CAROL CRISTINA; COSTA, MARIANE ROVERO; GREGOLIN, CRISTINA SCHMITT; MORETO, FERNANDO; FERREIRA, ANA LUCIA A.; MINATEL, IGOR OTAVIO; CORREA, CAMILA RENATA. Basic Concepts on the Role of Nuclear Factor Erythroid-Derived 2-Like 2 (Nrf2) in Age-Related Diseases. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 20, n. 13, p. 14-pg., 2019-07-01. (18/15294-3)

FABIANE VALENTINI FRANCISQUETI-FERRON; ARTUR JUNIO TOGNERI FERRON; ALESSANDRA ALTOMARE; JÉSSICA LEITE GARCIA; FERNANDO MORETO; ANA LÚCIA A. FERREIRA; IGOR OTÁVIO MINATEL; GIANCARLO ALDINI; CAMILA RENATA CORRÊA. Gama-orizanol reduz a inflamação e o estresse oxidativo nos rins pela modulação do eixo RAGE/AGEs em animais submetidos a uma dieta rica em gordura e açúcar. J. Bras. Nefrol., v. 43, n. 4, p. 460-469, 2021-06-18. (15/10626-0, 18/15294-3)

GARCIA, JESSICA LEITE; VILEIGAS, DANIELLE FERNANDES; GREGOLIN, CRISTINA SCHMITT; COSTA, MARIANE ROVERO; FRANCISQUETI-FERRON, FABIANE VALENTINI; TOGNERI FERRON, ARTUR JUNIO; SALOME DE CAMPOS, DIJON HENRIQUE; MORETO, FERNANDO; MINATEL, IGOR OTAVIO; ZANATI BAZAN, SILMEIA GARCIA; et al. ice (Oryza sativa L.) bran preserves cardiac function by modulating pro-inflammatory cytokines and redox state in the myocardium from obese rat. EUROPEAN JOURNAL OF NUTRITION, v. 61, n. 2, OCT 2021. (18/15294-3)