Thermosensitive organogels as strategies for the treatment of inflammatory processes: from supramolecular structure to pharmacological evaluation

• Grant number: 19/20303-4
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2020
• End date: October 31, 2022
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Daniele Ribeiro de Araujo
• Grantee: Daniele Ribeiro de Araujo
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
• Associated researchers: Cristina Padula ; Fabiano Yokaichiya ; Mirela Inês de Sairre ; Patrizia Santi ; Vinicius de Andrade Oliveira

Abstract

The drug administration on skin offers numerous advantages compared to traditional pathways such as local drug release, easy application and patient compliance, avoiding first-pass biotransformation. However, the stratum corneum complex structural organization is one of the main limitations to drug absorption. On the other hand, morphofunctional changes on stratum corneum are responsible for triggering the synthesis and release of inflammatory cytokines, highlighting the clinical profile of one of the most incident chronic inflammatory dermatoses, Atopic Dermatitis (AD), subject of this proposal. Considering the skin alterations, it is emphasized the need for evaluating the effectiveness of two phytochemicals, such as sulforaphane (SFN) and curcumin (CUR), as well as to develop new carriers systems making them available as possible new therapeutic strategies for the treatment of AD. The technological approach of this proposal refers to the development of different formulations based on lipid-poloxamer organogels, nanostructured colloidal systems that present an oil phase dispersed in an aqueous phase, as tools for the application of phytochemicals to the skin. The different compositions proposed as oil phase were designed to allow the incorporation of high concentrations of hydrophobic molecules (as CUR), as well as to modulate the permeation rate of amphiphilic molecules (such as SFN). One of the differential factors of the proposal is the use of aqueous phase constituted by binary systems with poloxamers (PL) that present different values of hydrophilic-lipophilic balances, being possible to investigate their supramolecular organization by high resolution physicochemical techniques (such as SANS, for example). In addition, the organogels high adhesion capacity to the skin, forming an uniform film, increases the formulation contact time with the application site. Taken together, the factors presented here underscore the need for investigations into the physicochemical characterization of organogels and their interactions with the lipid matrix components of the stratum corneum, enabling the study of the relationship between drug permeation mechanisms and possible structural changes observed in the stratum corneum. In addition to physicochemical assays, the evaluation of biocompatibility, molecular mechanisms and pharmacological effects in vitro and in vivo models, which mimic skin changes in the presence of Atopic Dermatitis, underscores the potential for pharmacotherapeutic and technological innovation of this proposal. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VIGATO, ARYANE A.; MACHADO, IAN P.; DEL VALLE, MATHEUS; DA ANA, PATRICIA A.; SEPULVEDA, ANDERSON F.; YOKAICHIYA, FABIANO; FRANCO, MARGARETH K. K. D.; LOIOLA, MESSIAS C.; TOFOLI, GIOVANA R.; CEREDA, CINTIA MARIA S.; et al. onoketonic Curcuminoid-Lidocaine Co-Deliver Using Thermosensitive Organogels: From Drug Synthesis to Epidermis Structural Studie. HARMACEUTIC, v. 14, n. 2, FEB 2022. (19/20303-4, 16/18045-9, 19/14773-8)

GUILGER-CASAGRANDE, MARIANA; DE BARROS, CECILIA T.; ANTUNES, VITORIA A. N.; DE ARAUJO, DANIELE R.; LIMA, RENATA. Perspectives and Challenges in the Fight Against COVID-19: The Role of Genetic Variability. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, MAR 15 2021. (19/20303-4, 20/05816-2, 17/13328-5)

RAMOS CAMPOS, ESTEFANIA VANGELIE; PROENCA, PATRICIA LUIZA DE FREITAS; DORETTO-SILVA, LORENA; ANDRADE-OLIVEIRA, VINICIUS; FRACETO, LEONARDO FERNANDES; DE ARAUJO, DANIELE RIBEIRO. Trends in nanoformulations for atopic dermatitis treatment. Expert Opinion on Drug Delivery, v. 17, n. 11, AUG 2020. (19/20303-4, 17/24402-1, 19/05100-0)

CAMPOS, ESTEFANIA V. R.; PROENCA, PATRICIA L. F.; DA COSTA, TAIS GERMANO; DE LIMA, RENATA; HEDTRICH, SARAH; FRACETO, LEONARDO FERNANDES; DE ARAUJO, DANIELE RIBEIRO. Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applications. ACS APPLIED POLYMER MATERIALS, v. 3, n. 9, p. 4436-4449, SEP 9 2021. (19/05100-0, 19/20303-4, 17/24402-1)

DE ARAUJO, DANIELE RIBEIRO; PADULA, CRISTINA. Topical Drug Delivery: Innovative Controlled Release Systems. PHARMACEUTICS, v. 15, n. 6, p. 3-pg., 2023-06-01. (19/20303-4)

CAMPOS, ESTEFANIA V. R.; PROENCA, PATRICIA L. F.; DA COSTA, TAIS G.; DE LIMA, RENATA; FRACETO, LEONARDO F.; DE ARAUJO, DANIELE R.. Using Chitosan-Coated Polymeric Nanoparticles-Thermosensitive Hydrogels in association with Limonene as Skin Drug Delivery Strategy. BIOMED RESEARCH INTERNATIONAL, v. 2022, p. 18-pg., 2022-04-07. (19/05100-0, 17/24402-1, 19/20303-4)

PADULA, CRISTINA; MACHADO, IAN POMPERMAYER; VIGATO, ARYANE ALVES; DE ARAUJO, DANIELE RIBEIRO. ew Strategies for Improving Budesonide Skin Retentio. PHARMACEUTICS, v. 14, n. 1, JAN 2022. (19/20303-4, 19/14773-8)

MONTEIRO DO NASCIMENTO, MONICA HELENA; AMBROSIO, FELIPE NOGUEIRA; FERRARAZ, DEBORA CARAJILIASCOV; WINDISCH-NETO, HERMANN; QUEROBINO, SAMYR MACHADO; NASCIMENTO-SALES, MICHELLE; ALBERTO-SILVA, CARLOS; CHRISTOFFOLETE, MARCELO AUGUSTO; FRANCO, MARGARETH K. K. D.; KENT, BEN; et al. Sulforaphane-loaded hyaluronic acid-poloxamer hybrid hydrogel enhances cartilage protection in osteoarthritis models. Materials Science & Engineering C-Materials for Biological Applications, v. 128, SEP 2021. (19/20303-4, 14/14457-5, 15/14763-1)

SEPULVEDA, ANDERSON F.; KUMPGDEE-VOLLRATH, MONT; FRANCO, MARGARETH K. K. D.; YOKAICHIYA, FABIANO; DE ARAUJO, DANIELE R.. Supramolecular structure organization and rheological properties modulate the performance of hyaluronic acid-loaded thermosensitive hydrogels as drug-delivery systems. Journal of Colloid and Interface Science, v. 630, p. 13-pg., 2023-01-15. (14/14457-5, 19/20303-4)

DE CASTRO, KARINE CAPPUCCIO; COCO, JULIA CEDRAN; DOS SANTOS, ERICA MENDES; ATAIDE, JANAINA ARTEM; MARTINEZ, RENATA MILIANI; MONTEIRO DO NASCIMENTO, MONICA HELENA; PRATA, JOAO; LOPES DA FONTE, PEDRO RICARDO MARTINS; SEVERINO, PATRICIA; MAZZOLA, PRISCILA GAVA; et al. Pluronic (R) triblock copolymer-based nanoformulations for cancer therapy: A 10-year overview. JOURNAL OF CONTROLLED RELEASE, v. 353, p. 21-pg., 2022-12-17. (18/10799-0, 20/03727-2, 21/00319-3, 19/20303-4, 20/11333-4, 18/06475-4, 19/16169-0, 18/07707-6, 17/10789-1, 19/08549-8)