Nanoparticle of zinc oxide as a functional feed

• Grant number: 19/26042-8
• Support Opportunities: Research Projects - Thematic Grants
• Start date: October 01, 2020
• End date: September 30, 2025
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Helder Louvandini
• Grantee: Helder Louvandini
• Host Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Pesquisadores principais: Alessandro Francisco Talamini Do Amarante
• Associated researchers: Adibe Luiz Abdalla ; Ana Claudia Alexandre de Albuquerque ; Antonio Pinheiro Faciola ; Caroline Rymer ; Concepta Margaret Mcmanus Pimentel ; Débora Botéquio Moretti ; Elisabete Aparecida de Nadai Fernandes ; Francisco Peñagaricano ; Hudson Wallace Pereira de Carvalho ; Luciana Morita Katiki ; Mario Binelli ; Partha Pratim Ray ; Patricia Louvandini ; Rodrigo Giglioti
• Associated research grant(s): 21/05076-1 - Multi-user equipment approved in grant 2019/26042-8: Thermocicle. Gastrointestinal microbiota in sheep supplemented with zinc oxide nanoparticles, AP.EMU
• Associated scholarship(s): 24/18029-0 - INTERACTION OF ZINC OXIDE NANOPARTICLE SUPPLEMENTATION AND HAEMONCHUS CONTORTUS INFECTION ON THE RUMEN AND ABOMASUM MICROBIOTA IN SANTA INÊS LAMBS., BP.DR; 23/13656-3 - Determination of the size of the zinc oxide nanoparticle (NP-Zn) absorbed in sheep and in CACO-2 cells cultured in vitro., BP.TT; 23/00333-1 - Blood metabolic profile of sheep supplemented with oxide nanoparticles of zinc and infected by Haemonchus contortus, BP.IC; + associated scholarships 23/03105-0 - Influence of nanoparticle of zinc on the response of lambs infected with Haemonchus contortus., BP.PD; 23/00598-5 - Fecal evaluation of sheep supplemented with zinc oxide nanoparticles and infected by H. contortus, BP.IC; 21/03479-1 - Influence of zinc on innate and acquired immune response in Haemonchus contortus infected lambs, BP.PD; 21/03587-9 - Maternal-descending effect of supplementation of zinc nanoparticles (Zn-NP) in Santa Inês sheep on antimicrobial resistance and in vivo and in vitro reproduction, in the OMICS era, BP.PD; 20/14481-4 - In vitro evaluation of the physical-chemical behavior of zinc oxide nanoparticles in the ruminal, abomasal and intestinal liquid from sheep, BP.IC - associated scholarships

Abstract

In this project the validity of zinc oxide nanoparticle (Zn-NP) supplementation in ruminant as a functional feed will be investigated, integrating different areas of knowledge (inter and multidisciplinary) such as chemistry, microbiology and animal sciences (nutrology , parasitology, genetics, reproduction and immunology) using advances of the "Omics" era. In an inedited and innovative way, we intend to select Zn-NP with high bioavailability and greater permanence in the animal organism promoting better animal performance and a more efficient production system with the possibility of presenting a new form of Zn supplementation to the animals and product to be marketed. In the sustainable production of ruminants, the use of pasture as a source of ruminant feed is critical to maintaining ruminant health and promoting animal welfare, but in this scenario, infection by endoparasites is unavoidable. In addition, there is an intimate relationship between the gastrointestinal microbiota and ruminant performance. The objective of this project is to identify and characterize Zn-NP and its effect on nutrition, fetal programming, immune response, and reproduction, on the endoparasite Haemonchus contortus (egg, larva and adult), the gastrointestinal microbiota (taxonomic diversity, and antimicrobial resistance), and the interaction between them in sheep. This proposal brings together the Center for Nuclear Energy in Agriculture/USP, Institute of Biosciences/UNESP, Institute of Animal Science/IZ, Butantan Institute, Biology Institute/University of Brasilia, University of Reading/UK and University of Florida/USA. A series of in vitro and in vivo studies (detailed in the proposal) will be carried out in order to achieve the overall goal of the project. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GIGLIOTI, RODRIGO; DA SILVA FERREIRA, JORGE FREIRE; LUCIANI, GUILHERME FAVERO; LOUVANDINI, HELDER; OKINO, CINTIA HIROMI; NICIURA, SIMONE CRISTINA MEO; DE SENA OLIVEIRA, MARCIA CRISTINA; DO AMARANTE, ALESSANDRO FRANCISCO TALAMINI; KATIKI, LUCIANA MORITA. Potential of Haemonchus contortus first-stage larvae to characterize anthelmintic resistance through P-glycoprotein gene expression. Small Ruminant Research, v. 217, p. 6-pg., 2022-11-10. (19/26042-8, 18/19452-2)

LINS, JOSE GABRIEL GONSALVES; ALBUQUERQUE, ANA CLAUDIA A.; LOUVANDINI, HELDER; AMARANTE, ALESSANDRO F. T.. Immunohistochemistry analyses of the abomasal mucosa show differences in cellular-mediated immune responses to Haemonchus contortus infection in resistant and susceptible young lambs. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, v. 161, p. 9-pg., 2024-08-31. (21/03479-1, 19/26042-8)