Food deprivation effects in kisspeptin neurons excitability

• Grant number: 19/21707-1
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2020
• End date: August 31, 2022
• Field of knowledge: Biological Sciences - Physiology
• Principal Investigator: Renata Frazão
• Grantee: Renata Frazão
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

It is well known that nutritional status affects directly the reproduction, since an adequate amount of energy is necessary for the onset of puberty, establishment of estral cyclicity and fertility. The regulation of food intake and energy balance are modulated by leptin, which acts mainly on the arcuate nucleus (ARH). Leptin modulates the activity of orexigenic neurons, neuropeptide Y and Agouti-related peptide, and anorexigenic neurons, pro-opiomelanocortin and Cocaine- and amphetamine-regulated transcript-expressing cells. Evidence suggests the interaction between kisspeptin neurons, the main activator of neurons that synthesize gonadotrophin releasing hormone, and neurons that regulate food intake. However, the mechanisms by which this interaction occurs are still not fully understood. In the present study we will use neuroanatomical and electrophysiological strategies additionally to genetically modified mice to evaluate whether the activity of kisspeptin neurons and consequently the reproductive system can be modulated by factors that alter food intake, such as fasting or caloric restriction. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE SOUZA, GABRIEL O.; CHAVES, FERNANDA M.; SILVA, JOSIANE N.; PEDROSO, JOAO A. B.; METZGER, MARTIN; FRAZAO, RENATA; DONATO JR, JOSE. Gap junctions regulate the activity of AgRP neurons and diet-induced obesity in male mice. Journal of Endocrinology, v. 255, n. 2, p. 16-pg., 2022-11-01. (21/11551-4, 19/21707-1, 17/21854-9, 20/01318-8, 17/16473-6)

DONATO, JR., JOSE; WASINSKI, FREDERICK; FURIGO, ISADORA C.; METZGER, MARTIN; FRAZAO, RENATA. Central Regulation of Metabolism by Growth Hormone. CELLS, v. 10, n. 1, JAN 2021. (20/01318-8, 16/09679-4, 19/21707-1, 16/20897-3, 17/16473-6)

WASINSKI, FREDERICK; TAVARES, MARIANA R.; GUSMAO, DANIELA O.; LIST, EDWARD O.; KOPCHICK, JOHN J.; ALVES, GUILHERME A.; FRAZAO, RENATA; DONATO JR, JOSE. Central growth hormone action regulates neuroglial and proinflammatory markers in the hypothalamus of male mice. Neuroscience Letters, v. 806, p. 8-pg., 2023-04-11. (19/21707-1, 19/07005-4, 20/10102-9, 20/01318-8, 21/03316-5)

ZIMERMAN, JEANINI; NINO, OSCAR M. S.; DA COSTA, CHARLES S.; ZANOL, JORDANA F.; COMERIO, MILENA; DE SOUZA, LETICIA N. DA GAMA; MIRANDA-ALVES, LEANDRO; MIRANDA, ROSIANE A.; LISBOA, PATRICIA C.; CAMILO, TAYS A.; et al. Subacute high-refined carbohydrate diet leads to abnormal reproductive control of the hypothalamic-pituitary axis in female rats. REPRODUCTIVE TOXICOLOGY, v. 119, p. 13-pg., 2023-05-29. (16/17968-6, 19/21707-1)

BOHLEN, TABATA M.; DE PAULA, DANIELLA G.; TEIXEIRA, PRYSCILA D. S.; MANSANO, NAIRA DA SILVA; ALVES, GUILHERME ANDRADE; DONATO JR, JOSE; FRAZAO, RENATA. Socs3 ablation in kisspeptin cells partially prevents lipopolysaccharide-induced body weight loss. CYTOKINE, v. 158, p. 9-pg., 2022-08-17. (15/14588-5, 19/21707-1, 20/01318-8, 13/00801-3, 16/03082-6, 17/22189-9)

QUARESMA, PAULA G. F.; WASINSKI, FREDERICK; MANSANO, NAIRA S.; FURIGO, ISADORA C.; TEIXEIRA, PRYSCILA D. S.; GUSMAO, DANIELA O.; FRAZAO, RENATA; DONATO JR, JOSE. Leptin Receptor Expression in GABAergic Cells is Not Sufficient to Normalize Metabolism and Reproduction in Mice. Endocrinology, v. 162, n. 11, NOV 2021. (21/03316-5, 16/09679-4, 19/21707-1, 17/25281-3, 20/01318-8, 17/22189-9, 16/20897-3)

MOINHO, TAIS M.; TAVARES, MARIANA R.; CAMPOS, ANA M. P.; FRAZAO, RENATA; METZGER, MARTIN; DONATO, JOSE, JR.. Simple method to induce denaturation of fluorescent proteins in free-floating brain slices. JOURNAL OF NEUROSCIENCE METHODS, v. 371, p. 10-pg., 2022-02-15. (19/21707-1, 20/01318-8, 20/10102-9, 17/16473-6)

CHAVES, FERNANDA M.; WASINSKI, FREDERICK; TAVARES, MARIANA R.; MANSANO, NAIRA S.; FRAZAO, RENATA; GUSMAO, DANIELA O.; QUARESMA, PAULA G. F.; PEDROSO, JOAO A. B.; ELIAS, CAROL F.; LIST, EDWARD O.; et al. Effects of the Isolated and Combined Ablation of Growth Hormone and IGF-1 Receptors in Somatostatin Neurons. Endocrinology, v. 163, n. 5, p. 13-pg., 2022-05-01. (16/20897-3, 17/25281-3, 20/01318-8, 20/10102-9, 19/21707-1, 17/21854-9, 17/22189-9, 21/03316-5)

GUSMAO, DANIELA O.; VIEIRA, HENRIQUE R.; MANSANO, NAIRA S.; TAVARES, MARIANA R.; DE SOUSA, LIGIA M. M.; WASINSKI, FREDERICK; FRAZAO, RENATA; DONATO JR, JOSE. Pattern of gonadotropin secretion along the estrous cycle of C57BL/6 female mice. PHYSIOLOGICAL REPORTS, v. 10, n. 17, p. 18-pg., 2022-09-01. (16/20897-3, 20/01318-8, 20/10102-9, 19/21707-1, 17/22189-9, 21/03316-5)