From the natural history to the conservation of Brazilian amphibinas

• Grant number: 22/11096-8
• Support Opportunities: Research Projects - Thematic Grants
• Start date: August 01, 2023
• End date: July 31, 2028
• Field of knowledge: Biological Sciences - Zoology
• Principal Investigator: Luis Felipe de Toledo Ramos Pereira
• Grantee: Luis Felipe de Toledo Ramos Pereira
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• City of the host institution: Campinas
• Associated researchers: Benjamin Timothy Phalan ; Carlos Guilherme Becker ; Célio Fernando Baptista Haddad ; Cybele Sabino Lisboa ; Daniel Loebmann ; David Chambers Blackburn Jr ; Diego José Santana Silva ; Elaine Maria Lucas Gonsales ; Juliane Silberschmidt Freitas ; Márcio Borges Martins ; Mario Ribeiro de Moura ; Rodrigo Lingnau ; Susana Oliveira Dias ; Thaís Barreto Guedes da Costa
• Associated scholarship(s): 25/02286-6 - In situ and laboratory analysis of the effects of environmental contamination on genotoxic, physiological, and morphological parameters of Rhinella diptycha in Fernando de Noronha, BP.DR; 25/12245-5 - Curation of biological collections at LaHNAB and assistance with field expeditions., BP.TT; 25/04907-8 - Sequencing and Bioinformatics of Amphibians and their Pathogens, BP.TT; + associated scholarships 25/01347-1 - Histological, Genotoxic, and Chemical Effects of Emerging Stressor Integration in Native (Rhinella icterica) and Invasive (Aquarana catesbeiana) Anurans, BP.DR; 24/09081-8 - Historical and geographic distribution, natural history and population genetics applied to the conservation of the fruit tree frog, Xenohyla truncata, BP.DR; 23/10830-2 - Ecological niche modeling and passive acoustic monitoring of three endemic and important species for the conservation of Atlantic Forest biodiversity, BP.DR; 23/16747-0 - COMPARATIVE ANALYSIS OF THE CARDIORESPIRATORY SYSTEM IN ANURANS, BP.DR; 24/10034-4 - Temporal Stability of Biodiversity Conservation Strategies, BP.MS; 24/10747-0 - Temporal trends in the description of global amphibian species, BP.IC; 24/10748-7 - Temporal trends in the discovery of smal mammals and songbirds globally, BP.IC; 24/06465-0 - Testing morphological evidence for recognition and description of a new anuran species, BP.IC; 24/00049-4 - Review of threats to amphibians in Brazil, BP.PD; 23/17980-0 - Pumpkin toadlets and the disappearance of amphibians, BP.JC; 23/17981-6 - pumpkin toadlets and the disappearance of amphibians, BP.JC; 23/17812-0 - The chytrid fungus and its influence on anuran vocalization., BP.IC; 23/11870-8 - Sequencing and Bioinformatics of Amphibians and their Pathogens, BP.TT; 23/11398-7 - Organization of the LaHNAB biological collections and support for field expeditions and lab experiments, BP.TT - associated scholarships

Abstract

The study of amphibian conservation is urgent and broad. In order to understand and act in the preservation of this taxon, we must deepen the studies in the different facets of the amphibian biology, from the most basic questions, such as taxonomy, behavior, natural history, ecology and evolution, but also to investigate the aspects more directly related to their threats, such as environmental changes (fragmentation and climate change), lethal pathologies and invasive species. It is in this integrative context that the present proposal fits. Our study team is multidisciplinary and must investigate different aspects of the biology of amphibians (essentially anurans), always with conservation in the background. In this project, we also foresee the collection and storage of a large amount of biological material (animals, audio and video recordings, photographs, cultures of the chytrid fungus, skin swabs and tissues for molecular analyses) that will be available to the Brazilian scientific community even after the end of the project. At the same time, in addition to producing data and scientific articles, we also propose the production of outreach material (a book of the Brazilian amphibians), so that the results can be used in other types of production, and also we will be transferring the knowledge generated in this project to the non-academic community. Still, unprecedented in our group, we should also investigate the interaction between humans and amphibians, to also better understand the Brazilian population and enhance their engagement in amphibian conservation. The project also provides for the training of human resources to work with the theme of conservation, in different lines of research, especially in studies with chytridiomycosis (still lacking dedicated laboratories in Brazil), with invasive species of amphibians, or even with animal communication, where we will address novel (or rarely explored) lines in Brazil (for example, the one focused on the hearing of frogs, and not on sound production, as more commonly addressed). Thus, this project is ambitious and innovative in several aspects and must, with the great commitment of a specialized team, contribute to issues ranging from natural history to amphibian conservation. (AU)

Scientific publications

(22)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CUNHA, MARIA EDUARDA B.; SOUZA, UBIRATA FERREIRA; MENDONCA, LUCAS R.; SILVA-SOARES, THIAGO; MALAGOLI, LEO R.; PEREIRA, DEIVID; FREIRE, MARCELO D.; COLOMBO, PATRICK. New records of phoresy of Elpidium (Ostracoda: Limnocytheridae) by anurans in the Brazilian Atlantic Forest. Phyllomedusa, v. 22, n. 2, p. 10-pg., 2023-12-01. (13/50741-7, 14/23677-9, 22/11096-8, 08/50928-1, 14/50342-8, 22/10081-7)

SOUZA, UBIRATA FERREIRA; MENDONCA, LUCAS ROSADO; CERON, KAROLINE; MENESES, AFONSO SANTIAGO DE OLIVEIRA; DE MOURA, GERALDO JORGE BARBOSA; DUBEUX, MARCOS J. MATIAS; TOLEDO, L. FELIPE. Frog eat frogs: the relationship among the Neotropical frogs of the genus Leptodactylus and their anuran prey. FOOD WEBS, v. 37, p. 8-pg., 2023-11-24. (19/18335-5, 22/10081-7, 20/12558-0, 22/11096-8)

BORGES, JAYNI SANTOS; GASPARINI, JOAO LUIZ; ALMEIDA, ANTONIO DE PADUA; TOLEDO, LUIS FELIPE; DENA, SIMONE. The advertisement call of Brachycephalus alipioi (Anura: Brachycephalidae), a pumpkin toadlet from Espirito Santo, Brazil. Zootaxa, v. 5689, n. 1, p. 7-pg., 2025-09-02. (22/11096-8)

BAGUIO, FRANK ALVIN GOZON; MACHADO, THAYNARA MENDES; HOLLAND, BRENDEN STEPHEN; TOLEDO, LUIS FELIPE. An off-key anuran singer: an unusual male release call in invasive cane toads in Hawai'i. Zootaxa, v. 5659, n. 4, p. 4-pg., 2025-07-07. (22/11096-8, 22/00437-9)

BOTELHO, LUCAS MACHADO; VERDADE, VANESSA KRUTH; TOLEDO, LUIS FELIPE; MUSCAT, EDELCIO. Spawning site plasticity in Cycloramphus boraceiensis (Anura: Cycloramphidae). Phyllomedusa, v. 23, n. 2, p. 6-pg., 2024-12-01. (22/11096-8)

OLIVEIRA, PATRICIA MARQUES DO A.; SOUZA, UBIRATA FERREIRA; DE SOUSA, JULIANA DELFINO; DE MELLO, ANNA VIRGINIA ALBANO; MAIA, NATALLIA VIVIAN NASCIMENTO DA SILVA; LIMA, JOSE HENRIQUE DE ANDRADE. Unraveling patterns and drivers of saurophagy in South American lizards. SCIENTIFIC REPORTS, v. 15, n. 1, p. 13-pg., 2025-02-22. (22/11096-8, 22/10081-7)

ERNETTI, JULIA R.; PRADO, JOELMA S.; TOLEDO, LUIS FELIPE. Host life stage susceptibility to the chytrid fungus in a Neotropical torrent frog. FUNGAL BIOLOGY, v. 129, n. 2, p. 8-pg., 2025-04-01. (22/11096-8, 16/25358-3, 20/02994-7, 19/18335-5)

PONTES, MARIANA RETUCI; ABADIE, MICHELLE; RIBEIRO, LUISA P.; AUGUSTO-ALVES, GUILHERME; MARTINS, MARCIO BORGES; BECKER, C. GUILHERME; TOLEDO, LUIS FELIPE. Climatic driver of chytrid prevalence in the Critically Endangered Admirable Redbelly Toad. PERSPECTIVES IN ECOLOGY AND CONSERVATION, v. 23, n. 3, p. 6-pg., 2025-07-30. (20/00099-0, 16/25358-3, 22/11096-8, 18/23622-0, 19/03170-0, 23/10166-5)

FAIVOVICH, JULIAN; C. LOURENCO, ANA CAROLINA; D. P. PINHEIRO, PAULO; L. LYRA, MARIANA; BAETA, DELIO; MAGALHAES, RAFAEL F.; GRANT, TARAN; L. PEZZUTI, TIAGO; S. F. LEITE, FELIPE; ARAUJO-VIEIRA, KATYUSCIA; et al. The phylogenetic relationships of Bokermanns treefrogs: species groups, reproductive biology, and biogeography (Anura: Hylidae: Bokermannohyla). CLADISTICS, v. 41, n. 4, p. 35-pg., 2025-06-23. (03/10335-8, 06/52088-5, 18/15425-0, 15/11237-7, 12/10000-5, 18/18473-6, 22/11096-8, 21/10639-5, 19/24979-2, 11/50146-6, 12/25370-2, 23/17996-3, 21/14476-3, 13/20423-3, 05/56756-0, 21/12455-9, 14/50342-8, 13/50741-7)

ERNETTI, JULIA R.; PRADO, JOELMA S.; RUGGERI, JOICE; BRASILEIRO, CINTHIA A.; TOLEDO, LUIS FELIPE. Chytrid fungus in threatened Brazilian amphibians: A focus on island populations. JOURNAL FOR NATURE CONSERVATION, v. 87, p. 8-pg., 2025-09-01. (19/18335-5, 20/02994-7, 22/11096-8, 20/12866-6)

BOVOLON, JOAO PEDRO; ARANHA, NATALIA; TAUCCI, PEDRO P. G.; NUNES-DE-ALMEIDA, CARLOS HENRIQUE L.; TOLEDO, LUIS FELIPE. A new population of the threatened torrent-frog Hylodes sazimai and other possible suitable sites based on ecological niche modeling. JOURNAL FOR NATURE CONSERVATION, v. 86, p. 8-pg., 2025-08-01. (23/10830-2, 22/11096-8, 23/11870-8)

TOLEDO, LUIS FELIPE; BOTELHO, LUCAS MACHADO; CARRASCO-MEDINA, ANDRES SANTIAGO; GRAY, JAIMI A.; ERNETTI, JULIA R.; GAMA, JOANA MOURA; LYRA, MARIANA LUCIO; BLACKBURN, DAVID C.; NUNES, IVAN; MUSCAT, EDELCIO. Among the world's smallest vertebrates: a new miniaturized flea-toad (Brachycephalidae) from the Atlantic rainforest. PeerJ, v. 12, p. 27-pg., 2024-10-25. (20/02994-7, 22/11096-8)

PEREIRA, ELVIS ALMEIDA; TOLEDO, LUIS FELIPE. Historical Range, Past Declines and Future Niche Suitability of Populations of Frogs of the Genus Dryadobates in the Atlantic Forest. TROPICAL CONSERVATION SCIENCE, v. 18, p. 11-pg., 2025-09-01. (22/11096-8, 24/00049-4)

ARANHA, NATALIA; REBOUCAS, RAONI; TOLEDO, LUIS FELIPE. The impact of superstitions on practices with Cururu toads in Brazil. Studies on Neotropical Fauna and Environment, v. N/A, p. 8-pg., 2025-03-12. (23/03090-2, 16/25358-3, 22/11096-8)

MACHADO, THAYNARA MENDES; DE-OLIVEIRA-NOGUEIRA, CARLOS HENRIQUE; SOUZA, UBIRATA FERREIRA; FIGUEIREDO-DE-ANDRADE, CAIO ANTONIO; MONICO, ALEXANDER TAMANINI; TOLEDO, LUIS FELIPE. The advertisement call of Xenohyla truncata (Anura: Hylidae), an endangered treefrog endemic to coastal Rio de Janeiro, Brazil. Zootaxa, v. 5351, n. 3, p. 5-pg., 2023-09-27. (16/25358-3, 22/10081-7, 19/18335-5, 22/11096-8)

DENA, SIMONE; ZORNOSA-TORRES, CAMILA; BOVOLON, JOAO PEDRO; PAIVA, FERNANDA; MACHADO, THAYNARA MENDES; DO PRADO, JOELMA SANTOS; ERNETTI, JULIA R.; CARRASCO-MEDINA, ANDRES SANTIAGO; DE ASSIS, CLODOALDO LOPES; MARINHO, RAYANNE VAZ DE MELLO; et al. Audiovisual collections safeguard undescribed calls: description of anuran vocalisations based on recordings from a public sound library. BIOACOUSTICS-THE INTERNATIONAL JOURNAL OF ANIMAL SOUND AND ITS RECORDING, v. 33, n. 5, p. 32-pg., 2024-08-05. (20/02994-7, 16/25358-3, 22/08274-1, 22/11096-8)

RIBEIRO, LUISA P.; RODRIGUEZ, DAVID; DOS SANTOS, ROSELI COELHO; LUCAS, ELAINE M.; TOLEDO, LUIS FELIPE. Genotypic Discrimination of Chytrid Fungus Lineages in the Amphibian Trade. ECOHEALTH, v. N/A, p. 12-pg., 2025-07-30. (22/11096-8, 18/23622-0, 20/02817-8)

AUGUSTO-ALVES, GUILHERME; ARANHA, NATALIA; TOLEDO, LUIS FELIPE; BASTOS, ROGERIO PEREIRA; LINGNAU, RODRIGO. Reproductive and agonistic interactions mediated by multimodal signals of a Neotropical torrent frog. ACTA ETHOLOGICA, v. N/A, p. 8-pg., 2025-07-18. (22/11096-8, 23/10166-5)

PEDROZO, MARIANA; BOTELHO, LUCAS MACHADO; DE TOLEDO MOROTI, MATHEUS; ROTENBERG, ELSIE LAURA; TOLEDO, LUIS FELIPE; MUSCAT, EDELCIO. Natural History of the Flattened Waterfall Frog Cycloramphus boraceiensis (Anura, Cycloramphidae). CALDASIA, v. 46, n. 1, p. 12-pg., 2024-01-01. (16/25358-3, 19/18335-5, 22/11096-8)

SOUZA, UBIRATA FERREIRA; AUGUSTO-ALVES, GUILHERME; PONTES, MARIANA RETUCI; BOTELHO, LUCAS MACHADO; MUSCAT, EDELCIO; TOLEDO, LUIS FELIPE. Ultrasonic distress calls and associated defensive behaviors in Neotropical frogs. ACTA ETHOLOGICA, v. 27, n. 2, p. 5-pg., 2024-01-08. (16/25358-3, 20/00099-0, 22/11096-8, 19/03170-0, 22/10081-7)

BOVOLON, JOAO PEDRO; TOLEDO, LUIS FELIPE. Dendropsophus minutus repertoire complexity and its relationship with environmental traits. BIOACOUSTICS-THE INTERNATIONAL JOURNAL OF ANIMAL SOUND AND ITS RECORDING, v. 33, n. 4, p. 16-pg., 2024-06-08. (16/25358-3, 22/11096-8)

RIBEIRO, LUISA P.; TOLEDO, LUIS FELIPE. Enhancing public policies for Brazilian bullfrog farming. Biota Neotropica, v. 25, n. 1, p. 12-pg., 2025-01-01. (22/11096-8, 16/25358-3, 18/23622-0)