High resolutions structural MRI and receptor imaging studies in refractory temporal lobe epilepsy: in vivo and ex vivo analyses

• Grant number: 05/56446-0
• Support Opportunities: Inter-institutional Cooperation in Support of Brain Research (CINAPCE) - Thematic Grants
• Start date: April 01, 2007
• End date: December 31, 2013
• Field of knowledge: Health Sciences - Medicine - Medical Radiology
• Principal Investigator: Edson Amaro Junior
• Grantee: Edson Amaro Junior
• Host Institution: Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE). Sociedade Beneficente Israelita Brasileira Albert Einstein (SBIBAE). São Paulo , SP, Brazil
• Pesquisadores principais: Carlos Alberto Moreira Filho
• Associated research grant(s): 09/10950-0 - 39th Annual Meeting of the Society for Neuroscience - Neuroscience 2009, AR.EXT
• Associated scholarship(s): 08/08902-5 - Radiotracers of Molecular Imaging in dopamine and serotonin receptors in animal model research in vivo SPECT compared with in vivo binding assay and ex vivo autoradiography., BP.MS

Abstract

This research proposal is centered in the study of refractory mesial temporal lobe epilepsy (MTLE) by high resolution structural MRI, receptor imaging and historadiological correlations. This approach will allow the development of methods for correlating in vivo and ex vivo imaging. Accordingly, we devised three projects that will investigate the basis of MRI signal alteration, serotonin transporters profile detected by molecular imaging and full analysis of brain parenchyma in patients with MTLE. The corresponding projects are histological basis of MRI signal in the temporal lobe (project A), molecular imaging in mesial temporal lobe epilepsy: in vivo and ex vivo quantification of serotonin transporters (project B) and whole brain high resolution mri analysis in patients with refractory mesial temporal lobe epilepsy (project C). The complete versions of projects A, B and C are on appendixes I, II and 3 respectively. The resources (researchers, technicians, imaging equipments) involved in the accomplishment of this proposal will make available to the ClnAPCe network: 1) a multidisciplinary team able to integrate clinical and experimental epileptology, innovative imaging acquisition/processing and advanced histopathological analysis; 2) a state-of-the-art core facility for advanced imaging studies; 3) new methodologies for historadiological correlations; 4) new tools for second order imaging processing; 5) expertise on molecular imaging. These technological and scientific resources are applicable to other important areas of biomedical research (oncology, neurodegenerative and psychiatric diseases) and are designed to be integrated into a network environment sharing knowledge with other centers via e-leaming. This is an essential requirement for the developing of telemedicine, a key tool for research, education and telediagnostic activities. In spite of the importance of epilepsy to public health, the number of researchers and clinically qualified specialists in the related areas of neuroscience remains small. The ClnAPCe network initiative is expected to promote a rapid progress in this field. Consequently, we have the responsibility for disseminating the knowledge generated in the network projects to the medical and scientific communities. For this reason, the main educational emphasis of our program is turned to technology transfer in imaging processing using voxel-based morphometry (VBM), molecular imaging acquisition techniques and neuroimage databasing and data mining. Since epilepsy is a disease that causes generalized anxiety in the public at large, we intend to provide a web site devoted to increase public awareness on the scientific advances achieved by the ClnAPCe projects. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AZEVEDO, HATYLAS; FUJITA, ANDRE; BANDO, SILVIA YUMI; IAMASHITA, PRISCILA; MOREIRA-FILHO, CARLOS ALBERTO. Transcriptional Network Analysis Reveals that AT1 and AT2 Angiotensin II Receptors Are Both Involved in the Regulation of Genes Essential for Glioma Progression. PLoS One, v. 9, n. 11, NOV 3 2014. (05/56446-0, 11/07762-8, 11/50761-2, 09/53443-1)

BANDO, SILVIA YUMI; BERTONHA, FERNANDA BERNARDI; PIMENTEL-SILVA, LUCIANA RAMALHO; MANSANO DE OLIVEIRA, JOAO GABRIEL; DUARTE CARNEIRO, MARCO ANTONIO; MANOEL OKU, MARIANA HIROMI; WEN, HUNG-TZU; MARTINS CASTRO, LUIZ HENRIQUE; MOREIRA-FILHO, CARLOS ALBERTO. Hippocampal CA3 transcriptional modules associated with granule cell alterations and cognitive impairment in refractory mesial temporal lobe epilepsy patients. SCIENTIFIC REPORTS, v. 11, n. 1, MAY 13 2021. (15/22308-2, 05/56446-0)

LIMA, FLAVIA A.; MOREIRA-FILHO, CARLOS A.; RAMOS, PATRICIA L.; BRENTANI, HELENA; LIMA, LEANDRO DE A.; ARRAIS, MAGALY; BENTO-DE-SOUZA, LUIZ C.; BENTO-DE-SOUZA, LUCIANA; DUARTE, MARIA I.; COUTINHO, ANTONIO; et al. Decreased AIRE Expression and Global Thymic Hypofunction in Down Syndrome. JOURNAL OF IMMUNOLOGY, v. 187, n. 6, p. 3422-3430, SEP 15 2011. (05/56446-0)

BANDO, SILVIA YUMI; SILVA, FILIPI NASCIMENTO; COSTA, LUCIANO DA FONTOURA; SILVA, ALEXANDRE V.; PIMENTEL-SILVA, LUCIANA R.; CASTRO, LUIZ H. M.; WEN, HUNG-TZU; AMARO, JR., EDSON; MOREIRA-FILHO, CARLOS ALBERTO. Complex Network Analysis of CA3 Transcriptome Reveals Pathogenic and Compensatory Pathways in Refractory Temporal Lobe Epilepsy. PLoS One, v. 8, n. 11, NOV 21 2013. (05/56446-0, 05/00587-5, 11/50761-2, 09/53443-1)

MOREIRA-FILHO, CARLOS ALBERTO; BANDO, SILVIA YUMI; BERTONHA, FERNANDA BERNARDI; IAMASHITA, PRISCILA; SILVA, FILIPI NASCIMENTO; COSTA, LUCIANO DA FONTOURA; SILVA, ALEXANDRE VALOTTA; MARTINS CASTRO, LUIZ HENRIQUE; WEN, HUNG-TZU. Community Structure Analysis of Transcriptional Networks Reveals Distinct Molecular Pathways for Early- and Late-Onset Temporal Lobe Epilepsy with Childhood Febrile Seizures. PLoS One, v. 10, n. 5, MAY 26 2015. (05/56446-0, 05/00587-5, 11/50761-2, 09/53443-1)